我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

PLAU ELISA 试剂盒

Name: PLAU ELISA 试剂盒
SKU: ABIN411365
Availability: OutOfStock

This 人 PLAU ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 PLAU. This ELISA Kit has been cited in 6+ publications.

ELISA

1 image

(6 references)

产品编号 ABIN411365

发货至: 中国

Quick Overview for PLAU ELISA 试剂盒 (ABIN411365)

抗原

See all PLAU ELISA试剂盒

PLAU (Plasminogen Activator, Urokinase (PLAU))

抗原表位

AA 1-431

适用

人

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

62.5-4000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Discover our top product PLAU ELISA Kit

最低检测浓度

62.5 pg/mL

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human uPA/PLAU

品牌

PicoKine™

Analytical Method

Quantitative

特异性

Expression system for standard: NSO
Immunogen sequence: M1-L431

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

<5pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: NSO
Immunogen sequence: M1-L431
Plasminogen Activator, Urokinase (PLAU) ELISA Kit
PLAU 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Tissue Homogenate

Plasminogen Activator, Urokinase (PLAU) ELISA Kit
PLAU 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum

Plasminogen Activator, Urokinase (PLAU) ELISA Kit
PLAU 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate, Urine

Plasminogen Activator, Urokinase (PLAU) ELISA Kit
PLAU 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate

Plasminogen Activator, Urokinase (PLAU) ELISA Kit
PLAU 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate

Plasminogen Activator, Urokinase (PLAU) ELISA Kit
PLAU 适用: 猴 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate

Plasminogen Activator, Urokinase (PLAU) ELISA Kit
PLAU 适用: 马 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate

Plasminogen Activator, Urokinase (PLAU) ELISA Kit
PLAU 适用: 兔 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate

Plasminogen Activator, Urokinase (PLAU) ELISA Kit
PLAU 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Plasminogen Activator, Urokinase (PLAU) ELISA Kit
PLAU 适用: Cow Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

说明

Sequence similarities: Belongs to the peptidase S1 family.
Tissue Specificity: Expressed in the prostate gland and prostate cancers. .

板类型

Pre-coated

实验流程

human uPA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for uPA has been precoated onto 96-well plates. Standards(NSO, M1-L431) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for uPA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human uPA amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human uPA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human uPA standard solution and each sample be measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(pg/ml): 126, Standard deviation: 5.92, CV(%): 4.7
- Sample 2: n=16, Mean(pg/ml): 1895, Standard deviation: 64.43, CV(%): 3.4
- Sample 3: n=16, Mean(pg/ml): 2619, Standard deviation: 138.8, CV(%): 5.3,
- Sample 1: n=24, Mean(pg/ml): 250, Standard deviation: 13.5, CV(%): 5.4
- Sample 2: n=24, Mean(pg/ml): 1568, Standard deviation: 95.65, CV(%): 6.1
- Sample 3: n=24, Mean(pg/ml): 2755, Standard deviation: 187.3, CV(%): 6.8
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
Bi, Zeng, Zhao, Wei, Yu, Wang, Yu, Cao, Shan, Wei: "miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBPα." in: Molecular therapy. Nucleic acids, Vol. 5, Issue 9, pp. e368, (2016) (PubMed).

Bronckaers, Hilkens, Fanton, Struys, Gervois, Politis, Martens, Lambrichts: "Angiogenic properties of human dental pulp stem cells." in: PLoS ONE, Vol. 8, Issue 8, pp. e71104, (2013) (PubMed).

Kim, Lee, Choi, Yoo, Yang: "Implication of MMP-9 and urokinase plasminogen activator (uPA) in the activation of pro-matrix metalloproteinase (MMP)-13." in: Rheumatology international, Vol. 32, Issue 10, pp. 3069-75, (2012) (PubMed).

Jiang, Zhang, Zhou, Zhang, Li, Zhang, Zhang, Qiao, Kong, Ma, Chen: "Relationships of uPA and VEGF expression in esophageal cancer and microvascular density with tumorous invasion and metastasis." in: Asian Pacific journal of cancer prevention : APJCP, Vol. 13, Issue 7, pp. 3379-83, (2012) (PubMed).

Wang, Li, Han, Li, Mi, Sun, Song, Han, Zhao, Wang: "Integrin ?3 and its ligand regulate the expression of uPA through p38 MAPK in breast cancer." in: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 118, Issue 12, pp. 909-17, (2010) (PubMed).

Yang, Shi, Li, Yi: "Effects of shRNA targeting maspin on invasion of gastric carcinoma SGC7901 cell line." in: Oncology reports, Vol. 25, Issue 1, pp. 259-65, (2010) (PubMed).
抗原 See all PLAU ELISA试剂盒

PLAU (Plasminogen Activator, Urokinase (PLAU))

别名

PLAU

背景

Protein Function: Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.
Background: Plasminogen activator,urokinase(PLAU, uPA) converts plasminogen to plasmin. Plasmin is involved in processing of amyloid precursor protein and degrades secreted and aggregated amyloid-beta, a hallmark of Alzheimer disease(AD). Urokinase has a molecular mass of about 54 kD and is composed of 2 disulfide-linked chains, A and B, of molecular masses 18 kD and 33 kD, respectively. It localized on 10q24. uPA facilitates cell migration by localizing proteolisys on the cell surface and by inducing intracellular signalling pathways. In human vascular smooth muscle cell(VSMC), uPA stimulates migration via the uPA receptor(uPAR) signalling complex containing TYK2 and phosphatidylinositol 3-kinase(PI3-K).
Synonyms: Urokinase-type plasminogen activator,U-plasminogen activator,uPA,3.4.21.73,Urokinase-type plasminogen activator long chain A,Urokinase-type plasminogen activator short chain A,Urokinase-type plasminogen activator chain B,PLAU,
Full Gene Name: Urokinase-type plasminogen activator
Cellular Localisation: Secreted.

基因ID

5328

UniProt

P00749

途径

Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration