Neurotrophin 3 ELISA 试剂盒
(3 references)Quick Overview for Neurotrophin 3 ELISA 试剂盒 (ABIN411340)
抗原
See all Neurotrophin 3 (NTF3) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.6 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat Neurotrophin-3
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: sf21
Immunogen sequence: Y139-T257 -
交叉反应 （详细）
- There is cross-reactivity with NT-4<2.5 % , and no detectable cross-reactivity with any other cytokine.
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预测反应
- Bovine,Canine,Chicken,Horse,Monkey,Rabbit
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灵敏度
- <2pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: sf21
Immunogen sequence: Y139-T257
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Neurotrophin 3 (NTF3) ELISA Kit
NTF3 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Neurotrophin 3 (NTF3) ELISA KitNTF3 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Neurotrophin 3 (NTF3) ELISA KitNTF3 适用: 兔 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Neurotrophin 3 (NTF3) ELISA KitNTF3 适用: Pig Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Neurotrophin 3 (NTF3) ELISA KitNTF3 适用: 小鸡 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Neurotrophin 3 (NTF3) ELISA KitNTF3 适用: 大鼠 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Neurotrophin 3 (NTF3) ELISA KitNTF3 适用: 人 AA 139-257 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Serum
Neurotrophin 3 (NTF3) ELISA KitNTF3 适用: 小鼠 AA 139-257 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Serum
Neurotrophin 3 (NTF3) ELISA KitNTF3 适用: 兔 AA 139-257 Colorimetric Sandwich ELISA
Neurotrophin 3 (NTF3) ELISA KitNTF3 适用: Cow AA 139-257 Colorimetric Sandwich ELISA
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Tissue Specificity: Brain and peripheral tissues.
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板类型
- Pre-coated
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实验流程
- rat Neurotrophin-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Neurotrophin-3 has been precoated onto 96-well plates. Standards(sf21, Y139-T257) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Neurotrophin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Neurotrophin-3 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL rat Neurotrophin-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernatants or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat Neurotrophin-3 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 94, Standard deviation: 4.42, CV(%): 4.7
- Sample 2: n=16, Mean(pg/ml): 382, Standard deviation: 20.63, CV(%): 5.4
- Sample 3: n=16, Mean(pg/ml): 624, Standard deviation: 37.44, CV(%): 6,
- Sample 1: n=24, Mean(pg/ml): 123, Standard deviation: 6.4, CV(%): 5.2
- Sample 2: n=24, Mean(pg/ml): 417, Standard deviation: 24.2, CV(%): 5.8
- Sample 3: n=24, Mean(pg/ml): 716, Standard deviation: 50.84, CV(%): 7.1
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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: "Combination of electroacupuncture and grafted mesenchymal stem cells overexpressing TrkC improves remyelination and function in demyelinated spinal cord of rats." in: Scientific reports, Vol. 5, pp. 9133, (2016) (PubMed).
: "Enhanced expression of neurotrophic factors in the injured spinal cord through vaccination with myelin basic protein-derived peptide pulsed dendritic cells." in: Spine, Vol. 40, Issue 2, pp. 95-101, (2015) (PubMed).
: "Improvement in nerve regeneration through a decellularized nerve graft by supplementation with bone marrow stromal cells in fibrin." in: Cell transplantation, Vol. 23, Issue 1, pp. 97-110, (2014) (PubMed).
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: "Combination of electroacupuncture and grafted mesenchymal stem cells overexpressing TrkC improves remyelination and function in demyelinated spinal cord of rats." in: Scientific reports, Vol. 5, pp. 9133, (2016) (PubMed).
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- Neurotrophin 3 (NTF3)
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别名
- NTF3
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背景
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Protein Function: Seems to promote the survival of visceral and proprioceptive sensory neurons.
Background: Neurotrophin-3(NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3(Ntrk3, also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest(NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.
Synonyms: Neurotrophin-3,NT-3,HDNF,Nerve growth factor 2,NGF-2,Neurotrophic factor,Ntf3,Ntf-3,
Full Gene Name: Neurotrophin-3
Cellular Localisation: Secreted. -
基因ID
- 81737
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UniProt
- P18280
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途径
- RTK signaling, Neurotrophin Signaling Pathway
抗原 See all Neurotrophin 3 (NTF3) ELISA试剂盒
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