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INHBA ELISA 试剂盒

Name: INHBA ELISA 试剂盒
SKU: ABIN411255
Availability: OutOfStock

This 小鼠 INHBA ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 INHBA. This ELISA Kit has been cited in1 publication.

ELISA

1 image

(1 reference)

产品编号 ABIN411255

发货至: 中国

Datasheet as PDF

Quick Overview for INHBA ELISA 试剂盒 (ABIN411255)

抗原

See all INHBA ELISA试剂盒

INHBA (Inhibin, beta A (INHBA))

抗原表位

AA 311-426

适用

小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

15.6-1000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva

Discover our top product INHBA ELISA Kit

最低检测浓度

15.6 pg/mL

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Activin A

品牌

PicoKine™

Analytical Method

Quantitative

特异性

Expression system for standard: CHO
Immunogen sequence: G311-S426

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

预测反应

Bovine,Monkey,Rabbit

灵敏度

<10pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: CHO
Immunogen sequence: G311-S426
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Inhibin, beta A (INHBA) ELISA Kit
INHBA 适用: 人 AA 311-426 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Saliva, Serum

Inhibin, beta A (INHBA) ELISA Kit
INHBA 适用: 大鼠 AA 311-426 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Saliva, Serum

Inhibin, beta A (INHBA) ELISA Kit
INHBA 适用: Cow AA 311-426 Colorimetric Sandwich ELISA

Inhibin, beta A (INHBA) ELISA Kit
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Inhibin, beta A (INHBA) ELISA Kit
INHBA 适用: Pig AA 311-426 Colorimetric Sandwich ELISA

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

说明

Tissue Specificity: Uterus, ovary and liver.

板类型

Pre-coated

实验流程

mouse Activin A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Activin A has been precoated onto 96-well plates. Standards(CHO, G311-S426) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for Activin A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Activin A amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse Activin A standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin, EDTA) or saliva to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Activin A standard solution and each sample be measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(pg/ml): 95, Standard deviation: 4.8, CV(%): 5
- Sample 2: n=16, Mean(pg/ml): 289, Standard deviation: 15.0, CV(%): 5.2
- Sample 3: n=16, Mean(pg/ml): 453, Standard deviation: 23.6, CV(%): 5.2,
- Sample 1: n=24, Mean(pg/ml): 98, Standard deviation: 7.8, CV(%): 8
- Sample 2: n=24, Mean(pg/ml): 301, Standard deviation: 18.7, CV(%): 6.2
- Sample 3: n=24, Mean(pg/ml): 495, Standard deviation: 24.3, CV(%): 4.9
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
Zhang, Ju, Nie, Song, Xu, Gao: "Adenovirus‑mediated knockdown of activin A receptor type 2A attenuates immune‑induced hepatic fibrosis in mice and inhibits interleukin‑17‑induced activation of primary hepatic stellate cells." in: International journal of molecular medicine, Vol. 42, Issue 1, pp. 279-289, (2018) (PubMed).
抗原 See all INHBA ELISA试剂盒

INHBA (Inhibin, beta A (INHBA))

别名

INHBA

背景

Protein Function: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
Background: Activin A is a homodimer of 14 kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules. Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types. Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa.
Synonyms: Inhibin beta A chain,Activin beta-A chain,Inhba,
Full Gene Name: Inhibin beta A chain
Cellular Localisation: Secreted.

基因ID

16323

UniProt

Q04998

途径

Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Autophagy