IRF3 ELISA 试剂盒
Quick Overview for IRF3 ELISA 试剂盒 (ABIN2866273)
抗原
See all IRF3 ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- DNA-binding ELISA that facilitate the study of transcription factor activation in mammalien tissue and cell culture extracts.
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品牌
- TransAM®
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Analytical Method
- Quantitative
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特异性
- The TransAM IRF-3 Kits are tested for sensitivity and specificity in detecting IRF-3 activation.
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产品特性
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Transcription factors are DNA-binding proteins that tightly regulate gene expression. They consist of two distinct domains - one that displays high affinity for a specific DNA sequence and one that confers transcriptional activity. Transcription factors are activated by phosphorylation of specific residues or by processing bound inhibitory proteins. Understanding and quantifying transcription factors is essential for the study of cell functions in relation to differentiation, brain activity, immune response, inflammation and various disease states. TransAM® Kits are sensitive, non-radioactive transcription factor ELISA kits that facilitate the study of transcription factor activation in mammalian tissue and cell extracts.
TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied. -
组件
- One or five 96-well plate(s) with plate sealer(s), primary antibody, HRP-conjugated secondary antibody, wild-type and mutated competitor oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions.
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Interferon Regulatory Factor 3 (IRF3) ELISA Kit
IRF3 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Interferon Regulatory Factor 3 (IRF3) ELISA KitIRF3 适用: 人 pSer386 Colorimetric Sandwich ELISA Cell Samples, Tissue Lysate
Interferon Regulatory Factor 3 (IRF3) ELISA KitIRF3 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Interferon Regulatory Factor 3 (IRF3) ELISA KitIRF3 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Interferon Regulatory Factor 3 (IRF3) ELISA KitIRF3 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Nuclear extracts prepared from untreated and poly (I-C) transfected Cos-7 cells were diluted down to 0.6 μg/well and assayed using the TransAM IRF-3 Kit. The ratio of the signals from the treated over the untreated cells must be above 4. Lot No. 33913005 was developed for 15 minutes. It gave a ratio of 20 (Figure 1). The relative activation of IRF-3 may vary depending on the cell type tested and the manner in which it was stimulated. TransAM IRF-3 assays are also tested for specificity. Kits are performed in the absence or presence of 20 pmol of competitor oligonucleotide that contains either a wild-type or mutated IRF consensus binding site (Figure 2). The wild-type oligonucleotide prevents IRF binding to the probe immobilized on the plate. Conversely, the mutated oligonucleotide has a limited effect on IRF binding.
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实验时间
- 5 h
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C/-80 °C
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储存方法
- Store the cell extract at -80°C. All other kit components can be stored at -20°C prior to first use. Then, we recommend storing the kit at 4°C except for the oligonucleotides, DTT, Protease Inhibitor Cocktail and Poly [d(I-C)] that should be kept at -20°C. This product is guaranteed for 6 months from date of receipt.
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有效期
- 6 months
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- IRF3 (Interferon Regulatory Factor 3 (IRF3))
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别名
- Irf-3
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途径
- TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades
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