TGFBI ELISA 试剂盒
(1 reference)Quick Overview for TGFBI ELISA 试剂盒 (ABIN2859321)
抗原
See all TGFBI ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse beta IG-H3/TGFBI
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
- NSO, G24-H683
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <5pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: G24-H683
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Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA Kit
TGFBI 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: 人 Colorimetric Sandwich ELISA 0.63 ng/mL - 40 ng/mL Cell Culture Supernatant, Plasma, Serum
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: 人 AA 24-683 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: 大鼠 AA 24-685 Colorimetric Sandwich ELISA
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: 小鼠 Colorimetric Sandwich ELISA 3 pg/mL-1000 ng/mL Cell Culture Supernatant, Plasma, Serum
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: Pig Colorimetric Sandwich ELISA 0.937 ng/mL - 60 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Tissue Specificity: Expressed in heart, kidney, liver, skeletal muscle, testis, thyroid and uterus (PubMed:8024701). .
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板类型
- Pre-coated
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实验流程
- mouse beta IG-H3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for beta IG-H3 has been precoated onto 96-well plates. Standards(NSO, G24-H683) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for beta IG-H3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse beta IG-H3 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse βIG-H3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse βIG-H3 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 447, Standard deviation: 28.61, CV(%): 6.4
- Sample 2: n=16, Mean(pg/ml): 1253, Standard deviation: 53.88, CV(%): 4.3
- Sample 3: n=16, Mean(pg/ml): 2346, Standard deviation: 115, CV(%): 4.9,
- Sample 1: n=24, Mean(pg/ml): 450, Standard deviation: 39.15, CV(%): 8.7
- Sample 2: n=24, Mean(pg/ml): 1238, Standard deviation: 64.38, CV(%): 5.2
- Sample 3: n=24, Mean(pg/ml): 2625, Standard deviation: 144.4, CV(%): 5.5
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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: "Characterization of bone marrow-derived mesenchymal stem cells from dimethyloxallyl glycine-preconditioned mice: Evaluation of the feasibility of dimethyloxallyl glycine as a mobilization agent." in: Molecular medicine reports, Vol. 13, Issue 4, pp. 3498-506, (2016) (PubMed).
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: "Characterization of bone marrow-derived mesenchymal stem cells from dimethyloxallyl glycine-preconditioned mice: Evaluation of the feasibility of dimethyloxallyl glycine as a mobilization agent." in: Molecular medicine reports, Vol. 13, Issue 4, pp. 3498-506, (2016) (PubMed).
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- TGFBI (Transforming Growth Factor, beta-Induced, 68kDa (TGFBI))
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别名
- TGFBI
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背景
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Protein Function: Plays a role in cell adhesion (PubMed:8024701). May play a role in cell-collagen interactions (By similarity). .
Background: Transforming growth factor, beta-induced, 68 kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion.
Synonyms: Transforming growth factor-beta-induced protein ig-h3,Beta ig-h3,Tgfbi,
Full Gene Name: Transforming growth factor-beta-induced protein ig-h3
Cellular Localisation: Secreted . Secreted, extracellular space, extracellular matrix . May be associated both with microfibrils and with the cell surface.. -
基因ID
- 21810
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UniProt
- P82198
抗原 See all TGFBI ELISA试剂盒
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