HMOX1 ELISA 试剂盒
(1 reference)Quick Overview for HMOX1 ELISA 试剂盒 (ABIN2859305)
抗原
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适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 93.7 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human HO-1/HMOX1
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
- E.coli, M1-M288
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: E.coli
Immunogen sequence: M1-M288
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Heme Oxygenase (Decycling) 1 (HMOX1) ELISA Kit
HMOX1 适用: 大鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Heme Oxygenase (Decycling) 1 (HMOX1) ELISA KitHMOX1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Heme Oxygenase (Decycling) 1 (HMOX1) ELISA KitHMOX1 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Heme Oxygenase (Decycling) 1 (HMOX1) ELISA KitHMOX1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Heme Oxygenase (Decycling) 1 (HMOX1) ELISA KitHMOX1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Heme Oxygenase (Decycling) 1 (HMOX1) ELISA KitHMOX1 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Heme Oxygenase (Decycling) 1 (HMOX1) ELISA KitHMOX1 适用: 人 Colorimetric Sandwich ELISA 93.7 pg/mL - 6000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Tissue Specificity: Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level). .
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板类型
- Pre-coated
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实验流程
- human HO-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for HO-1 has been precoated onto 96-well plates. Standards(E.coli, M1-M288) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HO-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human HO-1 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 375pg/mL, 187.5pg/mL, 93.7pg/m human HO-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human HO-1 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 527, Standard deviation: 19, CV(%): 3.6
- Sample 2: n=16, Mean(pg/ml): 2085, Standard deviation: 91.74, CV(%): 4.4
- Sample 3: n=16, Mean(pg/ml): 3561, Standard deviation: 185.2, CV(%): 5.2,
- Sample 1: n=24, Mean(pg/ml): 686, Standard deviation: 31.6, CV(%): 4.6
- Sample 2: n=24, Mean(pg/ml): 2664, Standard deviation: 151.8, CV(%): 5.7
- Sample 3: n=24, Mean(pg/ml): 3724, Standard deviation: 242.1, CV(%): 6.5
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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: "Effect of Blueberry Anthocyanins Malvidin and Glycosides on the Antioxidant Properties in Endothelial Cells." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 1591803, (2017) (PubMed).
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: "Effect of Blueberry Anthocyanins Malvidin and Glycosides on the Antioxidant Properties in Endothelial Cells." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 1591803, (2017) (PubMed).
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- HMOX1 (Heme Oxygenase (Decycling) 1 (HMOX1))
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别名
- HMOX1
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背景
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Protein Function: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.
Background: HMOX1 (heme oxygenase (decycling) 1) is a human gene that encodes for the enzyme heme oxygenase 1. It is localized to chromosome 22. Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.
Synonyms: Heme oxygenase 1,HO-1,1.14.99.3,HMOX1,HO, HO1,
Full Gene Name: Heme oxygenase 1
Cellular Localisation: Microsome . Endoplasmic reticulum membrane, Peripheral membrane protein, Cytoplasmic side. -
基因ID
- 3162
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UniProt
- P09601
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途径
- Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, SARS-CoV-2 Protein Interactome
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