IL17 Receptor B ELISA 试剂盒
Quick Overview for IL17 Receptor B ELISA 试剂盒 (ABIN2859296)
抗原
See all IL17 Receptor B (IL17RB) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-17RB
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: R18-K286 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: R18-K286
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Interleukin 17 Receptor B (IL17RB) ELISA Kit
IL17RB 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Interleukin 17 Receptor B (IL17RB) ELISA KitIL17RB 适用: 小鼠 Colorimetric Sandwich ELISA 0.2-50 ng/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 17 Receptor B (IL17RB) ELISA KitIL17RB 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Contains 1 SEFIR domain.
Tissue Specificity: Expressed in several endocrine tissues, mostly in fetal and adult liver, kidney, pancreas, testis, colon, brain and small intestine, not detected in peripheral blood leukocytes, lymphoid organs, and most cell lines.
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板类型
- Pre-coated
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实验流程
- human IL-17RB ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-17RB has been precoated onto 96-well plates. Standards(NSO, R18-K286) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17RB is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-17RB amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human IL-17RB standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL-17RB standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(ng/ml): 1.43, Standard deviation: 0.053, CV(%): 3.7
- Sample 2: n=16, Mean(ng/ml): 3.04, Standard deviation: 0.137, CV(%): 4.5
- Sample 3: n=16, Mean(ng/ml): 6.12, Standard deviation: 0.324, CV(%): 5.3,
- Sample 1: n=24, Mean(ng/ml): 1.86, Standard deviation: 0.106, CV(%): 5.7
- Sample 2: n=24, Mean(ng/ml): 3.43, Standard deviation: 0.233, CV(%): 6.8
- Sample 3: n=24, Mean(ng/ml): 6.51, Standard deviation: 0.469, CV(%): 7.2
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- IL17 Receptor B (IL17RB) (Interleukin 17 Receptor B (IL17RB))
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别名
- IL17RB
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背景
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Protein Function: Receptor for the proinflammatory cytokines IL17B and IL17E. May play a role in controlling the growth and/or differentiation of hematopoietic cells. .
Background: IL17RB, also known as CRL4, is a protein that in humans is encoded by the IL17RB gene. It is located on 3p21.1. IL17RB is produced by activated T cells exhibiting proinflammatory activities. The protein encoded by this gene is a cytokine receptor. This receptor specifically binds to IL17B and IL17E (IL25), but does not bind to IL17 (A) or IL17C. This receptor has been shown to mediate the activation of NF-kappaB and the production of IL8 induced by IL17E. And it may play a role in controlling the growth and/or differentiation of hematopoietic cells.
Synonyms: Interleukin-17 receptor B,IL-17 receptor B,IL-17RB,Cytokine receptor-like 4,IL-17 receptor homolog 1,IL-17Rh1,IL17Rh1,Interleukin-17B receptor,IL-17B receptor,IL17RB,CRL4, EVI27, IL17BR,UNQ2501/PRO19612,
Full Gene Name: Interleukin-17 receptor B
Cellular Localisation: Isoform 1: Cell membrane, Single-pass type I membrane protein. -
基因ID
- 55540
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UniProt
- Q9NRM6
抗原 See all IL17 Receptor B (IL17RB) ELISA试剂盒
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