IL17 Receptor B ELISA 试剂盒
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Quick Overview for IL17 Receptor B ELISA 试剂盒 (ABIN6720109)
抗原
See all IL17 Receptor B (IL17RB) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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Supplier Product No.
- koa0464
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Supplier
- Rockland
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原理
- Human IL-17RB Sandwich ELISA Kit for Quantitative Detection
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品牌
- AccuSignal™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: R18-K286 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Interleukin 17 Receptor B (IL17RB) ELISA Kit
IL17RB 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Interleukin 17 Receptor B (IL17RB) ELISA KitIL17RB 适用: 人 AA 18-286 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 17 Receptor B (IL17RB) ELISA KitIL17RB 适用: 小鼠 Colorimetric Sandwich ELISA 0.2 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 17 Receptor B (IL17RB) ELISA KitIL17RB 适用: 人 Colorimetric Sandwich ELISA 157-10000 pg/mL Cell Lysate, Plasma, Serum
Interleukin 17 Receptor B (IL17RB) ELISA KitIL17RB 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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说明
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Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human IL-17RB standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human IL-17RB standard solution and each sample be measured in duplicate.
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实验精密度
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Intra-Assay Precision (CV%): 4.8%, 4%, 7.1%
Inter-Assay Precision (CV%): 5.3%, 5%, 9% -
限制
- 仅限研究用
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- IL17 Receptor B (IL17RB) (Interleukin 17 Receptor B (IL17RB))
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别名
- IL-17RB
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背景
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Background: IL17RB, also known as CRL4, is a protein that in humans is encoded by the IL17RB gene. It is located on 3p21.1. IL17RB is produced by activated T cells exhibiting proinflammatory activities. The protein encoded by this gene is a cytokine receptor. This receptor specifically binds to IL17B and IL17E (IL25), but does not bind to IL17 (A) or IL17C. This receptor has been shown to mediate the activation of NF-kappaB and the production of IL8 induced by IL17E. And it may play a role in controlling the growth and/or differentiation of hematopoietic cells.
Gene Full Name: interleukin 17 receptor B
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基因ID
- 55540
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NCBI登录号
- NP_061195
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UniProt
- Q9NRM6
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