Complement Factor H ELISA 试剂盒
Quick Overview for Complement Factor H ELISA 试剂盒 (ABIN2859251)
抗原
See all Complement Factor H (CFH) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 1.56 ng/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Complement H/CFH
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
- NSO, S860-R1231
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <50pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: S860-R1231
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Complement Factor H (CFH) ELISA Kit
CFH 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Plasma, Serum
Complement Factor H (CFH) ELISA KitCFH 适用: 人 Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Plasma, Serum
Complement Factor H (CFH) ELISA KitCFH 适用: 小鼠 AA 875-1252 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Complement Factor H (CFH) ELISA KitCFH 适用: 大鼠 Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Plasma, Serum
Complement Factor H (CFH) ELISA KitCFH 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Complement Factor H (CFH) ELISA KitCFH 适用: 人 Colorimetric Sandwich ELISA 23.438 ng/mL - 1500 ng/mL Plasma, Serum, Tissue Homogenate
Complement Factor H (CFH) ELISA KitCFH 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 ng/mL - 1000 ng/mL Plasma, Serum, Tissue Homogenate
Complement Factor H (CFH) ELISA KitCFH 适用: 人 Colorimetric Sandwich ELISA 3.13 ng/mL - 200 ng/mL Cell Culture Supernatant, Plasma, Serum
Complement Factor H (CFH) ELISA KitWide Range CFH 适用: 人 Colorimetric Sandwich ELISA 23.44 ng/mL - 1500 ng/mL Plasma, Serum
Complement Factor H (CFH) ELISA KitCFH 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Milk, Plasma, Saliva, Serum, Urine
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Tissue Specificity: Expressed by the liver and secreted in plasma.
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板类型
- Pre-coated
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实验流程
- human CFH ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CFH has been precoated onto 96-well plates. Standards (NSO, S860-R1231) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CFH is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CFH amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human CFH standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CFH standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(ng/ml): 2.32, Standard deviation: 0.12, CV(%): 5.2
- Sample 2: n=16, Mean(ng/ml): 8.15, Standard deviation: 0.47, CV(%): 5.8
- Sample 3: n=16, Mean(ng/ml): 37.66, Standard deviation: 2.37, CV(%): 6.3,
- Sample 1: n=24, Mean(ng/ml): 2.01, Standard deviation: 0.13, CV(%): 6.9
- Sample 2: n=24, Mean(ng/ml): 9.00, Standard deviation: 0.56, CV(%): 6.3
- Sample 3: n=24, Mean(ng/ml): 45.39, Standard deviation: 3.22, CV(%): 7.1
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- Complement Factor H (CFH)
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别名
- CFH
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背景
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Protein Function: Factor H functions as a cofactor in the inactivation of C3b by factor I and also increases the rate of dissociation of the C3bBb complex (C3 convertase) and the (C3b)NBB complex (C5 convertase) in the alternative complement pathway.
Background: Complement factor H (CFH), originally known as beta-1H globulin, is a serum glycoprotein that regulates the function of the alternativecomplement pathway in fluid phase and on cellular surfaces. It binds to C3b, accelerates the decay of the alternative pathway convertase C3bBb, and also acts as a cofactor for complement factor I, another C3b inhibitor. The CFH gene is located on chromosome 1q32-q32.1 within a cluster of genes encoding the regulatory complement components of the activation of C3 (RCA for 'regulators of complement activation'). This gene cluster includes decay-accelerating factor (DAF), C4-binding protein (C4BPA and C4BPB), and the factor H-related genes CFHR1, CFHR2, CFHR3, CFHR4, and CFHR5, among others. The gene family has arisen by multiple duplication events.
Synonyms: Complement factor H,H factor 1,CFH,HF, HF1, HF2,
Full Gene Name: Complement factor H
Cellular Localisation: Secreted. -
基因ID
- 3075
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UniProt
- P08603
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途径
- Complement System, Cellular Response to Molecule of Bacterial Origin
抗原 See all Complement Factor H (CFH) ELISA试剂盒
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