Epiregulin ELISA 试剂盒
Quick Overview for Epiregulin ELISA 试剂盒 (ABIN2859248)
抗原
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适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Epiregulin
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
- E.coli, V60-L108
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: E.coli
Immunogen sequence: V60-L108
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Epiregulin (EREG) ELISA Kit
EREG 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Epiregulin (EREG) ELISA KitEREG 适用: 小鼠 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Plasma, Serum
Epiregulin (EREG) ELISA KitEREG 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Epiregulin (EREG) ELISA KitEREG 适用: Pig AA 60-108 Colorimetric Sandwich ELISA
Epiregulin (EREG) ELISA KitEREG 适用: 猴 AA 60-108 Colorimetric Sandwich ELISA
Epiregulin (EREG) ELISA KitEREG 适用: 小鼠 AA 53-101 Colorimetric Sandwich ELISA 125-8000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Epiregulin (EREG) ELISA KitEREG 适用: 小鼠 Colorimetric Sandwich ELISA 10-6000 pg/mL Cell Culture Supernatant, Plasma, Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Tissue Specificity: In normal adults, expressed predominantly in the placenta and peripheral blood leukocytes. High levels were detected in carcinomas of the bladder, lung, kidney and colon. .
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板类型
- Pre-coated
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实验流程
- human Epiregulin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Epiregulin has been precoated onto 96-well plates. Standards (E.coli,V60-L108) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Epiregulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Epiregulin amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Epiregulin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin , EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Epiregulin standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(ng/ml): 1.15, Standard deviation: 0.059, CV(%): 5.2
- Sample 2: n=16, Mean(ng/ml): 2.52, Standard deviation: 0.113, CV(%): 4.5
- Sample 3: n=16, Mean(ng/ml): 5.81, Standard deviation: 0.273, CV(%): 4.7,
- Sample 1: n=24, Mean(ng/ml): 1.63, Standard deviation: 0.096, CV(%): 5.9
- Sample 2: n=24, Mean(ng/ml): 2.87, Standard deviation: 0.146, CV(%): 5.1
- Sample 3: n=24, Mean(ng/ml): 5.66, Standard deviation: 0.350, CV(%): 6.2
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- Epiregulin (EREG)
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别名
- EREG
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背景
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Protein Function: Ligand of the EGF receptor/EGFR and ERBB4. Stimulates EGFR and ERBB4 tyrosine phosphorylation (PubMed:9419975). Contributes to inflammation, wound healing, tissue repair, and oocyte maturation by regulating angiogenesis and vascular remodeling and by stimulating cell proliferation (PubMed:24631357). .
Background: Epiregulin (EPR) is a protein that in humans is encoded by the EREG gene. It is a member of the epidermal growth factor family. Epiregulin can function as a ligand of epidermal growth factor receptor (EGFR), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. The secondary structure at the C-terminus epiregulin is different from other epidermal growth factor family ligands because of the lack of hydrogen bonds. The structural difference at the C-terminus may provide an explanation for the reduced binding affinity of epiregulin to the ERBB receptors.
Synonyms: Proepiregulin,Epiregulin,EPR,EREG,
Full Gene Name: Proepiregulin
Cellular Localisation: Epiregulin: Secreted, extracellular space. -
基因ID
- 2069
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UniProt
- O14944
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation
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