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COL18A1 ELISA 试剂盒

Name: COL18A1 ELISA 试剂盒
SKU: ABIN2859242
Availability: OutOfStock

This 小鼠 COL18A1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 COL18A1. This ELISA Kit has been cited in 3+ publications.

ELISA

1 image

(3 references)

产品编号 ABIN2859242

发货至: 中国

Datasheet as PDF

Quick Overview for COL18A1 ELISA 试剂盒 (ABIN2859242)

抗原

See all COL18A1 ELISA试剂盒

COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))

抗原表位

AA 1591-1774

适用

小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

156-10.000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

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最低检测浓度

156 pg/mL

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Endostatin

品牌

PicoKine™

Analytical Method

Quantitative

特异性

Expression system for standard: NSO
Immunogen sequence: H1591-K1774

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

<10pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: NSO
Immunogen sequence: H1591-K1774
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Collagen, Type XVIII, alpha 1 (COL18A1) ELISA Kit
COL18A1 适用: 大鼠 AA 1591-1774 Colorimetric Sandwich ELISA

Collagen, Type XVIII, alpha 1 (COL18A1) ELISA Kit
COL18A1 适用: 小鼠 Colorimetric Competition ELISA 0.5-10 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

说明

Sequence similarities: Belongs to the multiplexin collagen family.
Tissue Specificity: Expressed in liver, kidney, lung, skeletal muscle and testis. .

板类型

Pre-coated

实验流程

mouse Endostatin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Endostatin has been precoated onto 96-well plates. Standards(NSO, H1591-K1774) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Endostatin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Endostatin amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Endostatin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Endostatin standard solution and each sample be measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(ng/ml): 0.8, Standard deviation: 0.052, CV(%): 6.5
- Sample 2: n=16, Mean(ng/ml): 2.14, Standard deviation: 0.135, CV(%): 6.3
- Sample 3: n=16, Mean(ng/ml): 4.25, Standard deviation: 0.179, CV(%): 4.2,
- Sample 1: n=24, Mean(ng/ml): 0.84, Standard deviation: 0.06, CV(%): 7.1
- Sample 2: n=24, Mean(ng/ml): 2.23, Standard deviation: 0.147, CV(%): 6.6
- Sample 3: n=24, Mean(ng/ml): 4.37, Standard deviation: 0.253, CV(%): 5.8
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
Guo, Wu, Cen, Li, Kong, Zhou: "IL-22-producing Th22 cells play a protective role in CVB3-induced chronic myocarditis and dilated cardiomyopathy by inhibiting myocardial fibrosis." in: Virology journal, Vol. 11, Issue 1, pp. 2511, (2014) (PubMed).

Fu, Zhao, Wu, Wu, Xu, Liu, Tong: "Therapeutic mechanisms of Tongmai Dasheng Tablet on tripterygium glycosides induced rat model for premature ovarian failure." in: Journal of ethnopharmacology, Vol. 139, Issue 1, pp. 26-33, (2011) (PubMed).

Zhang, Qu, Cui, Guo, Zhang, Ma, Sun: "Combined endostatin and TRAIL gene transfer suppresses human hepatocellular carcinoma growth and angiogenesis in nude mice." in: Cancer biology & therapy, Vol. 8, Issue 5, pp. 466-73, (2009) (PubMed).
抗原 See all COL18A1 ELISA试剂盒

COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))

别名

Endostatin

物质类

Chemical

背景

Protein Function: Endostatin potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling.
Background: Endostatin is a naturally-occurring 20- kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And It is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.
Synonyms: Collagen alpha-1(XVIII) chain,Endostatin,Col18a1,
Full Gene Name: Collagen alpha-1(XVIII) chain
Cellular Localisation: Secreted, extracellular space, extracellular matrix.

基因ID

12822

UniProt

P39061