IL-34 ELISA 试剂盒
Quick Overview for IL-34 ELISA 试剂盒 (ABIN2859235)
抗原
See all IL-34 (IL34) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-34
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: N21-P235 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: E.coli
Immunogen sequence: N21-P235
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Interleukin 34 (IL34) ELISA Kit
IL34 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 34 (IL34) ELISA KitIL34 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 34 (IL34) ELISA KitIL34 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 34 (IL34) ELISA KitIL34 适用: 人 AA 21-242 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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板类型
- Pre-coated
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实验流程
- mouse IL-34 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-34 has been precoated onto 96-well plates. Standards(E.coli, N21-P235) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-34 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-34 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse IL-34 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse IL-34 standard solution and each sample is measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 675, Standard deviation: 28.35, CV(%): 4.2
- Sample 2: n=16, Mean(pg/ml): 1810, Standard deviation: 66.97, CV(%): 3.7
- Sample 3: n=16, Mean(pg/ml): 2944, Standard deviation: 103, CV(%): 3.5,
- Sample 1: n=24, Mean(pg/ml): 792, Standard deviation: 51.48, CV(%): 6.5
- Sample 2: n=24, Mean(pg/ml): 1948, Standard deviation: 103.2, CV(%): 5.3
- Sample 3: n=24, Mean(pg/ml): 3107, Standard deviation: 152.2, CV(%): 4.9
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- IL-34 (IL34) (Interleukin 34 (IL34))
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别名
- IL34
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背景
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Protein Function: Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1 (By similarity). .
Background: Interleukin-34, or IL-34 is a protein belonging to a group of cytokines called interleukins. It is mapped to 16q22.1. The discovery of IL-34 protein in the red pulp of the spleen suggests involvement in growth and development of myeloid cells, consistent with its activity on monocytes. IL-34 is a recently discovered cytokine that acts on tissue resident macrophages and Langerhans cells upon binding the receptor for CSF-1, CSF-1R. It also can promote the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor. In addition, it promotes the development, survival, and function of microglia and Langerhans cells, therefore, this cytokine may predominately function in brain and skin biology.
Synonyms: Interleukin-34,IL-34,Il34,
Full Gene Name: Interleukin-34
Cellular Localisation: Secreted. -
基因ID
- 76527
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UniProt
- Q8R1R4
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途径
- RTK signaling
抗原 See all IL-34 (IL34) ELISA试剂盒
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