FGF23 ELISA 试剂盒
Quick Overview for FGF23 ELISA 试剂盒 (ABIN2859231)
抗原
See all FGF23 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.6 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse FGF23
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
- E.coli, Y25-V251
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: E.coli
Immunogen sequence: Y25-V251
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Fibroblast Growth Factor 23 (FGF23) ELISA Kit
FGF23 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 23 (FGF23) ELISA KitFGF23 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 23 (FGF23) ELISA KitFGF23 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 23 (FGF23) ELISA KitFGF23 适用: 人 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Serum, Tissue Homogenate, Urine
Fibroblast Growth Factor 23 (FGF23) ELISA KitFGF23 适用: Pig Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 23 (FGF23) ELISA KitFGF23 适用: 犬 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 23 (FGF23) ELISA KitFGF23 适用: Cow Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
Fibroblast Growth Factor 23 (FGF23) ELISA KitFGF23 适用: 大鼠 Colorimetric Competition ELISA 2.47 pg/mL - 200 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 23 (FGF23) ELISA KitSmall Sample FGF23 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Growth Factor 23 (FGF23) ELISA KitFGF23 适用: 人 AA 25-251 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Tissue Specificity: Mainly expressed in the brain and thymus at low levels. In brain, preferentially expressed in the ventrolateral thalamic nucleus.
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板类型
- Pre-coated
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实验流程
- mouse FGF23 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for FGF23 has been precoated onto 96-well plates. Standards(E.coli, Y25-V251) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF23 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse FGF23 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse FGF23 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse FGF23 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 37.7, Standard deviation: 1.39, CV(%): 3.7
- Sample 2: n=16, Mean(pg/ml): 118, Standard deviation: 5.43, CV(%): 4.6
- Sample 3: n=16, Mean(pg/ml): 621, Standard deviation: 32.91, CV(%): 5.3,
- Sample 1: n=24, Mean(pg/ml): 38.2, Standard deviation: 2.56, CV(%): 6.7
- Sample 2: n=24, Mean(pg/ml): 128, Standard deviation: 8.70, CV(%): 6.8
- Sample 3: n=24, Mean(pg/ml): 642, Standard deviation: 49.43, CV(%): 7.7
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- FGF23 (Fibroblast Growth Factor 23 (FGF23))
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别名
- FGF23
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背景
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Protein Function: Regulator of phosphate homeostasis (By similarity). Inhibits renal tubular phosphate transport by reducing SLC34A1 levels (By similarity). Acts directly on the parathyroid to decrease PTH secretion (By similarity). Regulator of vitamin-D metabolism (By similarity). Negatively regulates osteoblasts differentiation and matrix mineralization (By similarity). Upregulates EGR1 expression in the presence of KL. .
Background: Fibroblast growth factor 23 or FGF23 is a protein that in humans is encoded by the FGF23 gene. It is a member of the fibroblast growth factor (FGF) family which is responsible for phosphate metabolism. The main function of FGF23 seems to be regulation of phosphate concentration in plasma. FGF23 is secreted by Osteocytes in response to elevated Calcitriol. And it acts on the kidneys, where it decreases the expression of NPT2, a sodium-phosphate cotransporter in the proximal tubule. Thus, FGF23 decreases the reabsorption and increases excretion of phosphate. Also, FGF23 may suppress 1-alpha-hydroxylase, reducing its ability to activate vitamin D and subsequently impairing calcium absorption.
Synonyms: Fibroblast growth factor 23,FGF-23,Fgf23,
Full Gene Name: Fibroblast growth factor 23
Cellular Localisation: Secreted . Secretion is dependent on O-glycosylation.. -
基因ID
- 64654
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UniProt
- Q9EPC2
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Negative Regulation of Hormone Secretion
抗原 See all FGF23 ELISA试剂盒
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