CDKN1B ELISA 试剂盒
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- 抗原 See all CDKN1B ELISA试剂盒
- CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Custom Mouse p27/Kip1 (CDKN1B) ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- The antibody pair provided in this kit recognizes Mouse p27/Kip1 (CDKN1B)
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) ELISA Kit
CDKN1B 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) ELISA KitCDKN1B 适用: 小鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) ELISA KitCDKN1B 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Lysate, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 实验流程
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Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all CDKN1B ELISA试剂盒
- CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))
- 别名
- p27/Kip1 (CDKN1B 产品)
- 别名
- CDKN4 ELISA Kit, KIP1 ELISA Kit, MEN1B ELISA Kit, MEN4 ELISA Kit, P27KIP1 ELISA Kit, cdkn1b ELISA Kit, sb:cb611 ELISA Kit, wu:fb63g10 ELISA Kit, wu:fb64g10 ELISA Kit, wu:fe18e03 ELISA Kit, CDKN1B ELISA Kit, Kip1 ELISA Kit, p27 ELISA Kit, FAM14D ELISA Kit, ISG12 ELISA Kit, ISG12A ELISA Kit, P27 ELISA Kit, Cdki1b ELISA Kit, AA408329 ELISA Kit, AI843786 ELISA Kit, p27Kip1 ELISA Kit, cdkn1bl ELISA Kit, cyclin dependent kinase inhibitor 1B ELISA Kit, cyclin-dependent kinase inhibitor 1Bb ELISA Kit, interferon alpha inducible protein 27 ELISA Kit, cyclin-dependent kinase inhibitor 1B ELISA Kit, cyclin-dependent kinase inhibitor 1Ba ELISA Kit, CDKN1B ELISA Kit, cdkn1bb ELISA Kit, IFI27 ELISA Kit, Cdkn1b ELISA Kit, cdkn1ba ELISA Kit
- 背景
- P27/Kip1 (CDKN1B)
- 基因ID
- 821314
- UniProt
- Q43735
- 途径
- Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy
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