P-Cadherin ELISA 试剂盒
Quick Overview for P-Cadherin ELISA 试剂盒 (ABIN2748467)
抗原
See all P-Cadherin (CDH3) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.041 ng/mL
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原理
- Rat P-Cadherin ELISA Kit for serum, plasma, and cell culture supernatant samples.
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Analytical Method
- Quantitative
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特异性
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: rat 4-1BB (TNFRSF9/CD137), Activin A, Adiponectin (ACRP30), CD80 (B7-1), CD48 (SLAMF2), CTACK, Decorin, Eotaxin-1, EphA5, Erythropoietin, FGF-BP, Flt-3 Ligand, Galectin-1, Galectin-3, Gas 1, GFR alpha-1 (GDNFR), gp130, HGF, IL-1 R6 (IL-1 Rrp2), IL-1 ra (IL-1 F3), IL-2 R alpha, IL-3, IL-7, IL-17F, IL-22, JAM-A, MIP-1 alpha, Neuropilin-1, Neuropilin-2, Nope, Notch-1, Notch-2, Prolactin, RANTES, SCF, TIM-1 (KIM-1), TIMP-2, TREM-1, TWEAK R.
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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P-Cadherin (CDH3) ELISA Kit
CDH3 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
P-Cadherin (CDH3) ELISA KitCDH3 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
P-Cadherin (CDH3) ELISA KitCDH3 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
P-Cadherin (CDH3) ELISA KitCDH3 适用: 小鼠 AA 100-647 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
P-Cadherin (CDH3) ELISA KitCDH3 适用: 人 AA 108-654 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
P-Cadherin (CDH3) ELISA KitCDH3 适用: 人 Colorimetric Sandwich ELISA 63-4000 pg/mL Cell Lysate, Plasma, Serum
P-Cadherin (CDH3) ELISA KitCDH3 适用: 人 Colorimetric Sandwich ELISA 20-18000 pg/mL Cell Culture Supernatant, Plasma, Serum
P-Cadherin (CDH3) ELISA KitCDH3 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Serum
P-Cadherin (CDH3) ELISA KitCDH3 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Serum
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应用备注
- Recommended Dilution for serum and plasma samples4 fold
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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实验流程
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Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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- P-Cadherin (CDH3)
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别名
- P-Cadherin
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背景
- P-Cadherin (CDH3 / Cadherin-3)
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UniProt
- Q9JIV6
抗原 See all P-Cadherin (CDH3) ELISA试剂盒
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