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IGDCC4 ELISA 试剂盒

This anti-IGDCC4 antibody (ABIN2748439) is a antibody detecting IGDCC4 in ELISA. Suitable for Rat.
产品编号 ABIN2748439
发货至: 中国

Quick Overview for IGDCC4 ELISA 试剂盒 (ABIN2748439)

抗原

See all IGDCC4 ELISA试剂盒
IGDCC4 (Immunoglobulin Superfamily, DCC Subclass, Member 4 (IGDCC4))

适用

  • 2
  • 1
大鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

应用范围

ELISA

样品类型

Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
  • 原理

    Custom Rat Neighbor of Punc E11 (IGDCC4) ELISA Kit.

    Analytical Method

    Quantitative

    特异性

    The antibody pair provided in this kit recognizes Rat Neighbor of Punc E11 (IGDCC4)

    产品特性

    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    组件

    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate

    试剂未包括

    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • 样本量

    100 μL

    板类型

    Pre-coated

    实验流程

    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.

    实验流程

    Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    限制

    仅限研究用
  • 储存条件

    -20 °C

    储存方法

    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

    有效期

    6 months
  • 抗原 See all IGDCC4 ELISA试剂盒

    IGDCC4 (Immunoglobulin Superfamily, DCC Subclass, Member 4 (IGDCC4))

    别名

    Nope

    背景

    Neighbor of Punc E11 (IGDCC4)

    UniProt

    B5DFA9
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