IGDCC4 ELISA 试剂盒
The 小鼠 IGDCC4 ELISA Kit (ABIN5510687) is a Colorimetric ELISA Kit designed to quantify 小鼠 IGDCC4.
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产品编号 ABIN5510687
发货至:
中国
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中国
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北京 101111
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Quick Overview for IGDCC4 ELISA 试剂盒 (ABIN5510687)
抗原
See all IGDCC4 ELISA试剂盒
IGDCC4
(Immunoglobulin Superfamily, DCC Subclass, Member 4 (IGDCC4))
抗原表位
AA 25-954
适用
小鼠
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
125 pg/mL - 8000 pg/mL
应用范围
ELISA
样品类型
Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
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最低检测浓度
- 125 pg/mL
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原理
- Sandwich Mouse NOPE/IGDCC4 ELISA Kit to quantitate Mouse Igdcc4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: E25-H954Capture antibody and Detection antibody: a monoclonal antibody from rat, a biotinylated polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 7.1%, 5.4%, 4.1%
Inter-Assay Precision (CV%): 7.6%, 7.2%, 4.2% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- IGDCC4 (Immunoglobulin Superfamily, DCC Subclass, Member 4 (IGDCC4))
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别名
- Igdcc4
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背景
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Background: IGDCC4, also known as NOPE, plays essential roles in biologic functions of the cell surface, including cell adhesion, cell migration, and signal transduction. NOPE is identified as an oncofetal marker of murine and human hepatocellular carcinoma (HCC). Its expression is elevated in mouse and human hepatoma cell lines and in mouse hepatomas, but it is not expressed in normal liver or preneoplastic mouse HCC. Furthermore, NOPE is expressed in both AFP -positive and -negative tumors and is specifically expressed in epithelial tumor cells.
Gene Full Name: immunoglobulin superfamily, DCC subclass, member 4
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基因ID
- 56741
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UniProt
- Q9EQS9
抗原 See all IGDCC4 ELISA试剂盒
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