MIF ELISA 试剂盒
Quick Overview for MIF ELISA 试剂盒 (ABIN2748336)
抗原
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检测方法
实验类型
应用范围
样品类型
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原理
- Custom Rhesus Macaque Macrophage-Migration Inhibitory Factor (MIF) ELISA Kit.
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Analytical Method
- Quantitative
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特异性
- The antibody pair provided in this kit recognizes Rhesus Macaque Macrophage-Migration Inhibitory Factor (MIF)
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA Kit
MIF 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: 兔 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitWide Range MIF 适用: 大鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: Pig Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: 人 AA 2-115 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Serum
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: 猴 AA 2-115 Colorimetric Sandwich ELISA
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) ELISA KitMIF 适用: 人 Colorimetric Sandwich ELISA 0.313-20 ng/mL Cell Culture Cells, Milk, Plasma, Saliva, Serum
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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实验流程
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Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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- MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))
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别名
- MIF
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背景
- Macrophage-Migration Inhibitory Factor (MIF)
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基因ID
- 574298
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UniProt
- Q6DN04
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途径
- Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
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