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IGFBP3 ELISA 试剂盒

Name: IGFBP3 ELISA 试剂盒
SKU: ABIN2748197
Availability: OutOfStock

This 猕猴 IGFBP3 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 猕猴 IGFBP3 in Plasma, Cell Culture Supernatant 和 Serum.

产品编号 ABIN2748197

发货至: 中国

Datasheet as PDF

Quick Overview for IGFBP3 ELISA 试剂盒 (ABIN2748197)

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See all IGFBP3 ELISA试剂盒

IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))

适用

猕猴

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

1.02 ng/mL

应用范围

ELISA

样品类型

Plasma, Cell Culture Supernatant, Serum

Discover our top product IGFBP3 ELISA Kit

最低检测浓度

1.02 ng/mL

原理

Rhesus Monkey IGFBP-3 ELISA Kit for serum, plasma, and cell culture supernatant samples.

Analytical Method

Quantitative

特异性

This ELISA antibody pair detects Rhesus Macaque IGFBP-3 and human IGFBP-3. Other species not determined.

产品特性
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
组件
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
试剂未包括
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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应用备注

Recommended Dilution for serum and plasma samples2 fold

样本量

100 μL

板类型

Pre-coated

实验流程
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.
实验流程

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

限制

仅限研究用
储存条件

-20 °C

储存方法

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

有效期

6 months
抗原 See all IGFBP3 ELISA试剂盒

IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))

别名

IGFBP-3

背景

Insulin-like Growth Factor Binding Protein 3 (IGFBP3)

UniProt

F6U2J6

途径

Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding