DKK1 ELISA 试剂盒
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Quick Overview for DKK1 ELISA 试剂盒 (ABIN2748048)
抗原
See all DKK1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 33 pg/mL
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原理
- Rat DKK-1 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
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Analytical Method
- Quantitative
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特异性
- This ELISA antibody pair detects Rat DKK-1 and Mouse DKK-1. Other species not determined.
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate< div class='ty-product-feature'>< div class='ty-product-feature__label'>Other Materials Required< div class='ty-product-feature__value'>
- Distilled or deionized water
- Precision pipettes to deliver 2 μl to 1 μl volumes
- Adjustable 1-25 μl pipettes for reagent preparation
- 100 μl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate< div class='ty-product-feature'>< div class='ty-product-feature__label'>Other Materials Required< div class='ty-product-feature__value'>
- Distilled or deionized water
- Precision pipettes to deliver 2 μl to 1 μl volumes
- Adjustable 1-25 μl pipettes for reagent preparation
- 100 μl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Dickkopf Homolog 1 (DKK1) ELISA Kit
DKK1 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Dickkopf Homolog 1 (DKK1) ELISA KitQuick DKK1 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Biological Fluids, Plasma, Serum
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 适用: 小鼠 AA 30-272 Colorimetric Sandwich ELISA 46.9 pg/mL - 3000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 适用: 人 AA 32-266 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Dickkopf Homolog 1 (DKK1) ELISA KitDKK1 适用: 大鼠 AA 23-270 Colorimetric Sandwich ELISA 46.9 pg/mL - 3000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
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应用备注
- Suggested Plasma Dilution: 2 fold
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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实验流程
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Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20°C.
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有效期
- 6 months
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- DKK1 (Dickkopf Homolog 1 (DKK1))
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别名
- Dkk-1
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背景
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Gene Symbols: DKK1, DKK1_PREDICTED, LOC100912050, NEWGENE_1307313, RCG_48380
Protein Name (Synonyms): Dickkopf WNT-signaling pathway inhibitor 1 (Dickkopf homolog 1 (Xenopus laevis)) (Dickkopf-like protein 1)
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基因ID
- 293897108348170, 293897
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UniProt
- D3Z9J1
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途径
- WNT signaling, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation
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