电话:: +86 (0512) 65829739
传真:: +86 (010) 6788 5057
电子邮件:: orders@antibodies-online.cn

我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

CD80 ELISA 试剂盒

This 大鼠 CD80 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 大鼠 CD80.

产品编号 ABIN2748018

发货至: 中国

Quick Overview for CD80 ELISA 试剂盒 (ABIN2748018)

抗原

See all CD80 ELISA试剂盒

CD80

适用

6
6
4
1
1
1
1
1
1
1
1
1

大鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

1.23-300 ng/mL

应用范围

ELISA

样品类型

Plasma, Cell Culture Supernatant, Serum

Discover our top product CD80 ELISA Kit

最低检测浓度

1.23 ng/mL

原理

Rat CD80 (B7-1) ELISA Kit for serum, plasma, and cell culture supernatant samples.

Analytical Method

Quantitative

特异性

Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: rat Activin A, Adiponectin, SLAMF2, CTACK, Decorin, Eotaxin-1, EphA5, EPO, FGF-BP, Flt-3L, Galectin-1, Galectin-3, Gas-1, GFRalpha-1, gp-130, HGF, IL-1R6, IL-1ra, IL-2Ralpha, IL-3, IL-7, IL-17F, IL-22, JAM-A, MIP-1alpha, Neuropilin-1, Neuropilin-2, Nope, Notch-1, Notch-2, P-Cadherin, Prolactin, RANTES, SCF, TIM-1 (KIM-1), TIMP-2, TREM-1, TWEAK R.

产品特性
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
组件
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
试剂未包括
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
CD80 (CD80) ELISA Kit
CD80 适用: 人 Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate

CD80 (CD80) ELISA Kit
CD80 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma, Serum

CD80 (CD80) ELISA Kit
CD80 适用: 小鼠 AA 37-245 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum

CD80 (CD80) ELISA Kit
CD80 适用: 人 AA 35-242 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum

CD80 (CD80) ELISA Kit
CD80 适用: 大鼠 AA 39-248 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum

CD80 (CD80) ELISA Kit
CD80 适用: 小鼠 Colorimetric Sandwich ELISA 28-7.000 pg/mL Cell Culture Supernatant, Plasma, Serum

CD80 (CD80) ELISA Kit
CD80 适用: 小鼠 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Plasma, Serum, Tissue Homogenate

CD80 (CD80) ELISA Kit
CD80 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Serum

CD80 (CD80) ELISA Kit
CD80 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Serum

CD80 (CD80) ELISA Kit
CD80 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Serum

应用备注

Recommended Dilution for serum and plasma samples2 fold

样本量

100 μL

板类型

Pre-coated

实验流程
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.
实验流程

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

限制

仅限研究用
储存条件

-20 °C

储存方法

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

有效期

6 months
抗原 See all CD80 ELISA试剂盒

CD80

别名

CD80

背景

CD80 (B7-1)

UniProt

Q62680

途径

TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints

You are here: