CD36 ELISA 试剂盒
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Quick Overview for CD36 ELISA 试剂盒 (ABIN2708472)
抗原
See all CD36 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 390 pg/mL
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原理
- Sandwich ELISA for quantitative detection of Mouse CD36 concentrations.
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Analytical Method
- Quantitative
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灵敏度
- 180 pg/mL
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组件
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- Coated 96-well Plate
- Standard
- Biotin-Labeled Detection Antibody (100X)
- Streptavidin-HRP (100X)
- Standard/Sample Diluent
- Detection Antibody Diluent
- Streptavidin-HRP Diluent
- Wash Buffer (20X)
- TMB Substrate Solution
- Stop Solution
- Plate Adhesive Strips
- Technical Manual
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm.
- Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
- Adjustable 1-25 ml pipettes for reagent preparation.
- 100 ml and 1 liter graduated cylinders.
- Absorbent paper.
- Distilled or deionized water.
- Computer and software for ELISA data analysis.
- Tubes to prepare standard or sample dilutions.
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CD36 (CD36) ELISA Kit
CD36 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD36 (CD36) ELISA KitCD36 适用: 小鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
CD36 (CD36) ELISA KitCD36 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
CD36 (CD36) ELISA KitCD36 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
CD36 (CD36) ELISA KitCD36 适用: 小鼠 AA 30-439 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
CD36 (CD36) ELISA KitCD36 适用: 大鼠 AA 30-439 Colorimetric Sandwich ELISA 218-14.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
CD36 (CD36) ELISA KitCD36 适用: 人 AA 30-439 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
CD36 (CD36) ELISA KitCD36 适用: 小鼠 Colorimetric Sandwich ELISA 30-20000 pg/mL Cell Culture Supernatant, Plasma, Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards
- Add 100 µL Standard or Sample
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Biotin-Labeled Detection Antibody Working Solution
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Steptavidin-HRP Working Solution
- Wash plate 5 times with Wash Buffer Working Solution
- Add 100 µL TMB Substrat Solution
- Add 100 µL Stop Solution
- Read the plate at 450 nm
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限制
- 仅限研究用
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注意事项
- Avoid freeze/thaw cycles
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储存条件
- 4 °C,-20 °C
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储存方法
- Shipped and store at 4°C for 6 months, store at -20°C for one year.
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有效期
- 6-12 months
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- CD36
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别名
- CD36
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背景
- Alternative Names: GP3B, GP4, Platelet glycoprotein 4, Fatty acid translocase, FAT, Glycoprotein IIIb, GPIIIB, Leukocyte differentiation antigen CD36, PAS IV, PAS-4, Platelet collagen receptor, Platelet glycoprotein IV, GPIV, Thrombospondin receptor, CD36
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基因ID
- 12491
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UniProt
- Q08857
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途径
- TLR signaling, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, Lipid Metabolism, S100 Proteins
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