Growth Hormone 1 ELISA 试剂盒
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Quick Overview for Growth Hormone 1 ELISA 试剂盒 (ABIN2708231)
抗原
See all Growth Hormone 1 (GH1) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich ELISA for quantitative detection of Human GH1 concentrations.
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Analytical Method
- Quantitative
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灵敏度
- 59 pg/mL
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组件
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- Coated 96-well Plate
- Standard
- Biotin-Labeled Detection Antibody (100X)
- Streptavidin-HRP (100X)
- Standard/Sample Diluent
- Detection Antibody Diluent
- Streptavidin-HRP Diluent
- Wash Buffer (20X)
- TMB Substrate Solution
- Stop Solution
- Plate Adhesive Strips
- Technical Manual
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm.
- Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
- Adjustable 1-25 ml pipettes for reagent preparation.
- 100 ml and 1 liter graduated cylinders.
- Absorbent paper.
- Distilled or deionized water.
- Computer and software for ELISA data analysis.
- Tubes to prepare standard or sample dilutions.
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Growth Hormone 1 (GH1) ELISA Kit
GH1 适用: 小鸡 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Growth Hormone 1 (GH1) ELISA KitGH1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Growth Hormone 1 (GH1) ELISA KitGH1 适用: Pig Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Growth Hormone 1 (GH1) ELISA KitGH1 适用: 犬 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Growth Hormone 1 (GH1) ELISA KitGH1 适用: Cow Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Growth Hormone 1 (GH1) ELISA KitGH1 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Growth Hormone 1 (GH1) ELISA KitGH1 适用: 犬 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Growth Hormone 1 (GH1) ELISA KitGH1 适用: 小鸡 Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Growth Hormone 1 (GH1) ELISA KitGH1 适用: 小鸡 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Growth Hormone 1 (GH1) ELISA KitGH1 适用: Cow Colorimetric Competition ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards
- Add 100 µL Standard or Sample
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Biotin-Labeled Detection Antibody Working Solution
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Steptavidin-HRP Working Solution
- Wash plate 5 times with Wash Buffer Working Solution
- Add 100 µL TMB Substrat Solution
- Add 100 µL Stop Solution
- Read the plate at 450 nm
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限制
- 仅限研究用
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注意事项
- Avoid freeze/thaw cycles
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储存条件
- 4 °C,-20 °C
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储存方法
- Shipped and store at 4°C for 6 months, store at -20°C for one year.
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有效期
- 6-12 months
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- Growth Hormone 1 (GH1)
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别名
- GH1
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背景
- Alternative Names: Somatotropin, Growth hormone, GH, GH-N, Growth hormone 1, Pituitary growth hormone
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基因ID
- 2688
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UniProt
- P01241
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途径
- NF-kappaB Signaling, JAK/STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process
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