FURIN ELISA 试剂盒
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Quick Overview for FURIN ELISA 试剂盒 (ABIN2708221)
抗原
See all FURIN ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich ELISA for quantitative detection of Human FURIN concentrations.
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Analytical Method
- Quantitative
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灵敏度
- 55 pg/mL
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组件
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- Coated 96-well Plate
- Standard
- Biotin-Labeled Detection Antibody (100X)
- Streptavidin-HRP (100X)
- Standard/Sample Diluent
- Detection Antibody Diluent
- Streptavidin-HRP Diluent
- Wash Buffer (20X)
- TMB Substrate Solution
- Stop Solution
- Plate Adhesive Strips
- Technical Manual
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试剂未包括
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- Microplate reader capable of measuring absorbance at 450 nm.
- Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
- Adjustable 1-25 ml pipettes for reagent preparation.
- 100 ml and 1 liter graduated cylinders.
- Absorbent paper.
- Distilled or deionized water.
- Computer and software for ELISA data analysis.
- Tubes to prepare standard or sample dilutions.
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Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN) ELISA Kit
FURIN 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN) ELISA KitFURIN 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN) ELISA KitFURIN 适用: 小鼠 Colorimetric Sandwich ELISA 46.875 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN) ELISA KitFURIN 适用: 人 Colorimetric Sandwich ELISA 23.5 pg/mL - 1500 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN) ELISA KitFURIN 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN) ELISA KitFURIN 适用: 人 AA 108-715 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN) ELISA KitFURIN 适用: 人 Colorimetric Sandwich ELISA 23.5-1500 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN) ELISA KitFURIN 适用: 人 Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Lysate, Cell Culture Supernatant, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards
- Add 100 µL Standard or Sample
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Biotin-Labeled Detection Antibody Working Solution
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Steptavidin-HRP Working Solution
- Wash plate 5 times with Wash Buffer Working Solution
- Add 100 µL TMB Substrat Solution
- Add 100 µL Stop Solution
- Read the plate at 450 nm
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限制
- 仅限研究用
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注意事项
- Avoid freeze/thaw cycles
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储存条件
- 4 °C,-20 °C
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储存方法
- Shipped and store at 4°C for 6 months, store at -20°C for one year.
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有效期
- 6-12 months
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- FURIN (Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN))
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别名
- FURIN
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背景
- Alternative Names: FUR, PACE, PCSK3, Furin, Dibasic-processing enzyme, Paired basic amino acid residue-cleaving enzyme, PACE
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基因ID
- 5045
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UniProt
- P09958
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途径
- Notch Signaling, Neurotrophin Signaling Pathway
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