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VEGFB ELISA 试剂盒

The 人 VEGFB ELISA Kit (ABIN2703565) is a Colorimetric ELISA Kit designed to quantify 人 VEGFB. This ELISA Kit has been cited in 1 publication.

ELISA

1 image

(1 reference)

产品编号 ABIN2703565

发货至: 中国

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Quick Overview for VEGFB ELISA 试剂盒 (ABIN2703565)

抗原

See all VEGFB ELISA试剂盒

VEGFB (Vascular Endothelial Growth Factor B (VEGFB))

适用

人

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

0.4 ng/mL - 100 ng/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma, Serum

Discover our top product VEGFB ELISA Kit

最低检测浓度

0.4 ng/mL

原理

Human VEGF-B ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Analytical Method

Quantitative

特异性

This ELISA antibody pair detects human VEGF-B. Other species not determined.

产品特性
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
组件
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
,
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
试剂未包括
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Vascular Endothelial Growth Factor B (VEGFB) ELISA Kit
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VEGFB 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate

Vascular Endothelial Growth Factor B (VEGFB) ELISA Kit
VEGFB 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate

Vascular Endothelial Growth Factor B (VEGFB) ELISA Kit
VEGFB 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate

Vascular Endothelial Growth Factor B (VEGFB) ELISA Kit
VEGFB 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Vascular Endothelial Growth Factor B (VEGFB) ELISA Kit
VEGFB 适用: 小鼠 AA 20-207 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum

Vascular Endothelial Growth Factor B (VEGFB) ELISA Kit
VEGFB 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Vascular Endothelial Growth Factor B (VEGFB) ELISA Kit
VEGFB 适用: 小鼠 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

应用备注

Suggested Plasma Dilution: 2 fold

板类型

Pre-coated

实验流程
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.
限制

仅限研究用
储存条件

-20 °C

储存方法

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

有效期

6 months
Liu, Xu, Jiao, Ren, Li: "Vascular endothelial growth factor B coordinates metastasis of non-small cell lung cancer." in: Tumour biology, Vol. 36, Issue 3, pp. 2185-91, (2015) (PubMed).
抗原 See all VEGFB ELISA试剂盒

VEGFB (Vascular Endothelial Growth Factor B (VEGFB))

别名

VEGF-B

背景

Gene Symbols: VEGFB, VRF
Protein Name (Synonyms): Vascular endothelial growth factor B (VEGF-B) (VEGF-related factor) (VRF)

基因ID

7423

UniProt

P49765

途径

RTK signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals