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RBP4 ELISA 试剂盒

RBP4 适用: 人 Colorimetric Sandwich ELISA 0.1-30 ng/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN2703438
发货至: 中国
  • 抗原 See all RBP4 ELISA试剂盒
    RBP4 (Retinol Binding Protein 4, Plasma (RBP4))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.1-30 ng/mL
    最低检测浓度
    0.1 ng/mL
    应用范围
    ELISA
    原理
    Human RBP4 ELISA Kit for cell culture supernatants, plasma, and serum samples.
    样品类型
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair detects human RBP4. This antibody pair does not cross-react with mouse RBP4.
    灵敏度
    0.1 ng/mL
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 应用备注
    Recommended Dilution for serum and plasma samples10,000 fold
    样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • Ghareeb, Khalil, Hafez, Bajorath, Ahmed, Sarhan, Elwakeel, El-Demellawy: "Berberine Reduces Neurotoxicity Related to Nonalcoholic Steatohepatitis in Rats." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 361847, (2015) (PubMed).

  • 抗原 See all RBP4 ELISA试剂盒
    RBP4 (Retinol Binding Protein 4, Plasma (RBP4))
    别名
    RBP-4 (RBP4 产品)
    别名
    RDCCAS ELISA Kit, RBP ELISA Kit, RNBP ELISA Kit, rbp4 ELISA Kit, MGC54038 ELISA Kit, srbp ELISA Kit, PRBP ELISA Kit, RBPA ELISA Kit, Rbp-4 ELISA Kit, fb23c12 ELISA Kit, rbp ELISA Kit, wu:fb23c12 ELISA Kit, wu:fb58d04 ELISA Kit, wu:fb72b04 ELISA Kit, zgc:86911 ELISA Kit, retinol binding protein 4 ELISA Kit, renin binding protein ELISA Kit, retinol binding protein 4 S homeolog ELISA Kit, retinol binding protein 4 A, plasma ELISA Kit, retinol binding protein 4, plasma ELISA Kit, retinol binding protein 4 L homeolog ELISA Kit, RBP4 ELISA Kit, RENBP ELISA Kit, rbp4.S ELISA Kit, rbp4 ELISA Kit, Rbp4 ELISA Kit, RBP4A ELISA Kit, rbp4.L ELISA Kit
    背景
    Gene Names: RBP4 PRO2222
    Protein names: Retinol-binding protein 4 (Plasma retinol-binding protein) (PRBP) (RBP) [Cleaved into: Plasma retinol-binding protein(1-182), Plasma retinol-binding protein(1-181), Plasma retinol-binding protein(1-179), Plasma retinol-binding protein(1-176)]
    基因ID
    5950
    UniProt
    P02753
    途径
    Regulatory RNA Pathways, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response
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