PGLYRP1 ELISA 试剂盒
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- 抗原 See all PGLYRP1 ELISA试剂盒
- PGLYRP1 (Peptidoglycan Recognition Protein 1 (PGLYRP1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 6-1.500 pg/mL
- 最低检测浓度
- 6 pg/mL
- 应用范围
- ELISA
- 原理
- Human PGRPs ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair detects human PGRPs, less than 0.05 % cross-reactivity with human PGRP-1 beta and mouse PGRPs is observed.
- 灵敏度
- 6 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Peptidoglycan Recognition Protein 1 (PGLYRP1) ELISA Kit
PGLYRP1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Peptidoglycan Recognition Protein 1 (PGLYRP1) ELISA KitPGLYRP1 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Peptidoglycan Recognition Protein 1 (PGLYRP1) ELISA KitPGLYRP1 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Peptidoglycan Recognition Protein 1 (PGLYRP1) ELISA KitPGLYRP1 适用: 小鼠 AA 19-182 Colorimetric Sandwich ELISA
Peptidoglycan Recognition Protein 1 (PGLYRP1) ELISA KitPGLYRP1 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Plasma, Serum
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- 应用备注
- Recommended Dilution for serum and plasma samples100 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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- 抗原 See all PGLYRP1 ELISA试剂盒
- PGLYRP1 (Peptidoglycan Recognition Protein 1 (PGLYRP1))
- 别名
- PGRPs (PGLYRP1 产品)
- 别名
- PGLYRP1 ELISA Kit, PGLYRP ELISA Kit, pgrp ELISA Kit, tag7 ELISA Kit, pgrps ELISA Kit, pglyrp ELISA Kit, pgrp-s ELISA Kit, tnfsf3l ELISA Kit, MGC108330 ELISA Kit, MGC108469 ELISA Kit, PGRP-SC1a ELISA Kit, si:dkey-113g17.4 ELISA Kit, si:ch211-202c21.3 ELISA Kit, PGRP ELISA Kit, PGRP-S ELISA Kit, PGRPS ELISA Kit, TAG7 ELISA Kit, TNFSF3L ELISA Kit, Pglyrp ELISA Kit, Tag7 ELISA Kit, Tasg7 ELISA Kit, Tnfsf3l ELISA Kit, Pgrp ELISA Kit, peptidoglycan recognition protein 1 ELISA Kit, peptidoglycan recognition protein 1 L homeolog ELISA Kit, peptidoglycan recognition protein 5 ELISA Kit, PGLYRP1 ELISA Kit, pglyrp1.L ELISA Kit, pglyrp1 ELISA Kit, pglyrp5 ELISA Kit, Pglyrp1 ELISA Kit
- 背景
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Gene Names: PGLYRP1 PGLYRP PGRP TNFSF3L SBBI68 UNQ639/PRO1269
Protein names: Peptidoglycan recognition protein 1 (Peptidoglycan recognition protein short) (PGRP-S)
- 基因ID
- 8993
- UniProt
- O75594
- 途径
- Activation of Innate immune Response
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