PGLYRP1 ELISA 试剂盒
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北京 101111
Quick Overview for PGLYRP1 ELISA 试剂盒 (ABIN8055871)
抗原
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适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 312 pg/mL
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原理
- Sandwich Human PGLYRP1 ELISA Kit to quantitate Human PGLYRP1 in cell culture supernatants, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: Q22-P196Capture antibody and Detection antibody: polyclonal antibody from goat|polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 150 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Peptidoglycan Recognition Protein 1 (PGLYRP1) ELISA Kit
PGLYRP1 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Peptidoglycan Recognition Protein 1 (PGLYRP1) ELISA KitPGLYRP1 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Peptidoglycan Recognition Protein 1 (PGLYRP1) ELISA KitPGLYRP1 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Peptidoglycan Recognition Protein 1 (PGLYRP1) ELISA KitPGLYRP1 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Peptidoglycan Recognition Protein 1 (PGLYRP1) ELISA KitPGLYRP1 适用: 小鼠 AA 19-182 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Peptidoglycan Recognition Protein 1 (PGLYRP1) ELISA KitPGLYRP1 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Peptidoglycan Recognition Protein 1 (PGLYRP1) ELISA KitPGLYRP1 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Plasma, Serum
Peptidoglycan Recognition Protein 1 (PGLYRP1) ELISA KitPGLYRP1 适用: 人 Colorimetric Sandwich ELISA 6-1.500 pg/mL Cell Culture Supernatant, Plasma, Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 2.9%, 4.8%, 3.7%
Inter-Assay Precision (CV%): 7.4%, 3.1%, 5.3% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- PGLYRP1 (Peptidoglycan Recognition Protein 1 (PGLYRP1))
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别名
- PGLYRP
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背景
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Background: Peptidoglycan recognition protein 1, PGLYRP1, also known as TAG7, is an antibacterial and pro-inflammatory innate immunity protein that in humans is encoded by the PGLYRP1 gene. Peptidoglycan recognition proteins (PGRPs) are molecules that recognize peptidoglycan, a large component in bacterial cell walls. In insects, PGRPs activate antimicrobial pathways, and in mammals PGRPs function as antibacterial neutrophil proteins. PGRP-L halts bacterial growth by acting as an alanine amidase, an enzyme that hydrolyzes the amide bond of bacterial peptidoglycan. PGRP-I and PGRP-I are also members of the PGRP family that help recognize bacteria by binding to peptidoglycan and Gram-positive bacteria, but they do not have amidase activity. PGRP-S participates in intracellular killing of Gram-positive bacteria by stimulating two antimicrobial defense systems, the prophenoloxidase cascade and the antimicrobial peptides through Toll receptors.
Gene Full Name: peptidoglycan recognition protein 1
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基因ID
- 8993
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UniProt
- O75594
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途径
- Activation of Innate immune Response
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