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Periostin ELISA 试剂盒

This 小鼠 Periostin ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 Periostin.

ELISA image for Periostin (POSTN) ELISA Kit (ABIN2703411) (Periostin ELISA 试剂盒)

1 image

产品编号 ABIN2703411

发货至: 中国

Quick Overview for Periostin ELISA 试剂盒 (ABIN2703411)

抗原

See all Periostin (POSTN) ELISA试剂盒

Periostin (POSTN)

适用

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小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

0.05-16 ng/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma, Serum

Discover our top product POSTN ELISA Kit

最低检测浓度

0.05 ng/mL

原理

Mouse Periostin ELISA Kit for cell culture supernatants, Heparin and/or EDTA treated plasma, and serum samples. Citrate is not recommended.

Analytical Method

Quantitative

特异性

This ELISA kit shows no cross-reactivity with the following cytokines tested: Axl, BLC, CD27 Ligand, CD30, CD30 Ligand, CD40, CXCL16, EGF, E-selectin, Fractalkine, GITR, HGF, ICAM-1, IFN-gamma, IGF-1, IGFBP-2, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 ra, IL-1 beta, IL-2, IL-2 R alpha, IL-3, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 p70, IL-17E, IL-17F, IL-20, IL-23, IL-28, I-TAC, MCP-1, MCP-5, M-CSF, MDC, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 alpha, Osteopontin, Osteoprotegerin, Prolactin, Pro-MMP-9, P-selectin, Resistin, SCF, SDF-1 alpha, TPO, VCAM-1.

灵敏度

0.05 ng/mL

产品特性
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
组件
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
试剂未包括
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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POSTN 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

应用备注

Recommended Dilution for serum and plasma samples50 - 500 fold

样本量

100 μL

板类型

Pre-coated

实验流程
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.
样品制备

Collect plasma using heparin and/or EDTA as the anticoagulant. Citrate is not recommended.

限制

仅限研究用
储存条件

-20 °C

储存方法

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

有效期

6 months
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Periostin (POSTN)

别名

Periostin

背景

Gene Names: Postn Osf2
Protein names: Periostin (PN) (Osteoblast-specific factor 2) (OSF-2)

基因ID

50706

UniProt

Q62009

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