Periostin ELISA 试剂盒
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北京 101111
Quick Overview for Periostin ELISA 试剂盒 (ABIN1889333)
抗原
See all Periostin (POSTN) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich Human Periostin/OSF2 ELISA Kit to quantitate Human POSTN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: N22-Q836Capture antibody and Detection antibody: monoclonal antibody from mouse, monoclonal antibody from mouse
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Periostin (POSTN) ELISA Kit
POSTN 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum
Periostin (POSTN) ELISA KitPOSTN 适用: 小鼠 Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum
Periostin (POSTN) ELISA KitPOSTN 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Periostin (POSTN) ELISA KitPOSTN 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Periostin (POSTN) ELISA KitPOSTN 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Periostin (POSTN) ELISA KitPOSTN 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Periostin (POSTN) ELISA KitPOSTN 适用: 大鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
Periostin (POSTN) ELISA KitPOSTN 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum
Periostin (POSTN) ELISA KitPOSTN 适用: 小鼠 AA 24-836 Colorimetric Sandwich ELISA 93.7 pg/mL - 6000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 4.6%, 7.3%, 4%
Inter-Assay Precision (CV%): 8.2%, 8.2%, 4.1% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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: "TGFβ3-mediated induction of Periostin facilitates head and neck cancer growth and is associated with metastasis." in: Scientific reports, Vol. 6, pp. 20587, (2016) (PubMed).
: "Dysregulation of astrocyte extracellular signaling in Costello syndrome." in: Science translational medicine, Vol. 7, Issue 286, pp. 286ra66, (2015) (PubMed).
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- Periostin (POSTN)
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别名
- POSTN
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背景
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Background: Periostin, also known as OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. Periostin functions as a ligand for alpha-V/beta-3 and alpha-V/beta-5 integrins to support adhesion and migration of epithelial cells. It is found that periostin was overexpressed by the majority of human primary breast cancers examined. After myocardial infarction, periostin-induced cardiomyocyte cell cycle reentry and mitosis were associated with improved ventricular remodeling and myocardial function, reduced fibrosis and infarct size, and increase angiogenesis.
Gene Full Name: periostin
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基因ID
- 10631
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UniProt
- Q15063
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