IL36A/IL1F6 ELISA 试剂盒
Quick Overview for IL36A/IL1F6 ELISA 试剂盒 (ABIN2703145)
抗原
See all IL36A/IL1F6 (IL1F6) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.8 ng/mL
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原理
- Human IL-36 alpha (IL-1 F6) ELISA Kit for cell culture supernatants, EDTA and/or Citrate treated plasma, and serum samples. Heparin is not recommended.
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Analytical Method
- Quantitative
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特异性
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human IL-1F1, IL-1F2, IL-1F3, IL-1F4, IL-1F5, IL-1F7, IL-1F8, IL-1F9, IL-1F10, IL-1F11.
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灵敏度
- 0.8 ng/mL
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Interleukin 1 Family, Member 6 (IL1F6) ELISA Kit
IL1F6 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Interleukin 1 Family, Member 6 (IL1F6) ELISA KitIL1F6 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Interleukin 1 Family, Member 6 (IL1F6) ELISA KitIL1F6 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Recommended Dilution for serum and plasma samples2 fold
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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样品制备
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Collect plasma using EDTA and/or Citrate as the anticoagulant. Heparin is not recommended.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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- IL36A/IL1F6 (IL1F6) (Interleukin 1 Family, Member 6 (IL1F6))
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别名
- IL-36 alpha
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背景
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Gene Names: IL36A FIL1E IL1E IL1F6
Protein names: Interleukin-36 alpha (FIL1 epsilon) (Interleukin-1 epsilon) (IL-1 epsilon) (Interleukin-1 family member 6) (IL-1F6)
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基因ID
- 27179
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UniProt
- Q9UHA7
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途径
- Cancer Immune Checkpoints
抗原 See all IL36A/IL1F6 (IL1F6) ELISA试剂盒
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