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IGFBP7 ELISA 试剂盒
IGFBP7
适用: 人
Colorimetric
Sandwich ELISA
0.065-50 ng/mL
Cell Culture Supernatant, Plasma, Serum
IGFBP7ELISA Kit详情
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IGFBP7
(Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))
适用
All reactivities for IGFBP7 ELISA试剂盒
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.065-50 ng/mL
最低检测浓度
0.065 ng/mL
应用范围
ELISA
原理
Human IGFBP-rp1 (IGFBP-7) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
样品类型
Cell Culture Supernatant, Plasma, Serum
Analytical Method
Quantitative
特异性
This ELISA antibody pair recognizes Human IGFBP-rp1. Other species not yet determined.
灵敏度
0.065 ng/mL
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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使用细节
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应用备注
Recommended Dilution for serum and plasma samples100 fold
样本量
100 μL
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
IGFBP7目标详情
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IGFBP7
(Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))
别名
IGFBP-Rp1/IGFBP-7 (IGFBP7 产品 )
别名
zgc:85888 ELISA Kit, AGM ELISA Kit, FSTL2 ELISA Kit, IBP-7 ELISA Kit, IGFBP-7 ELISA Kit, IGFBP-7v ELISA Kit, IGFBPRP1 ELISA Kit, MAC25 ELISA Kit, PSF ELISA Kit, RAMSVPS ELISA Kit, TAF ELISA Kit, Fstl2 ELISA Kit, Mac25 ELISA Kit, insulin like growth factor binding protein 7 ELISA Kit, insulin-like growth factor binding protein 7 ELISA Kit, IGFBP7 ELISA Kit, igfbp7 ELISA Kit, Igfbp7 ELISA Kit
背景
Gene Names: IGFBP7 MAC25 PSF Protein names: Insulin-like growth factor-binding protein 7 (IBP-7) (IGF-binding protein 7) (IGFBP-7) (IGFBP-rP1) (MAC25 protein) (PGI2-stimulating factor) (Prostacyclin-stimulating factor) (Tumor-derived adhesion factor) (TAF)
基因ID
3490
UniProt
Q16270
途径
Growth Factor Binding
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