IGFBP7 ELISA 试剂盒
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- 抗原 See all IGFBP7 ELISA试剂盒
- IGFBP7 (Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))
- 抗原表位
- AA 30-282
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 625-40000 pg/mL
- 最低检测浓度
- 625 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IGFBP7
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine
- Analytical Method
- Quantitative
- 特异性
-
Expression system for standard: NSO
Immunogen sequence: D30-L282 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <20pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
-
Expression system for standard: NSO
Immunogen sequence: D30-L282 - Featured
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Insulin-Like Growth Factor Binding Protein 7 (IGFBP7) ELISA Kit
IGFBP7 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 7 (IGFBP7) ELISA KitIGFBP7 适用: Pig Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 7 (IGFBP7) ELISA KitIGFBP7 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 7 (IGFBP7) ELISA KitIGFBP7 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Insulin-Like Growth Factor Binding Protein 7 (IGFBP7) ELISA KitIGFBP7 适用: Pig Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Insulin-Like Growth Factor Binding Protein 7 (IGFBP7) ELISA KitIGFBP7 适用: 猴 AA 30-282 Colorimetric Sandwich ELISA
Insulin-Like Growth Factor Binding Protein 7 (IGFBP7) ELISA KitIGFBP7 适用: 人 Colorimetric Sandwich ELISA 625 pg/mL - 40000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Urine
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
-
Sequence similarities: Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
- 板类型
- Pre-coated
- 实验流程
- human IGFBP7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP7 has been precoated onto 96-well plates. Standards(NSO, D30-L282) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IGFBP7 amount of sample captured in plate.
- 实验流程
-
Aliquot 0.1 mL per well of the 40000pg/mL, 20000pg/mL, 10000pg/mL, 5000pg/mL, 2,500pg/mL, 1,250pg/mL, 625pg/mL human IGFBP7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IGFBP7 standard solution and each sample be measured in duplicate.
- 实验精密度
-
- Sample 1: n=16, Mean(ng/ml): 2.3, Standard deviation: 0.101, CV(%): 4.4
- Sample 2: n=16, Mean(ng/ml): 16.2, Standard deviation: 0.729, CV(%): 4.5
- Sample 3: n=16, Mean(ng/ml): 28.1, Standard deviation: 1.43, CV(%): 5.1,
- Sample 1: n=24, Mean(ng/ml): 2.8, Standard deviation: 0.188, CV(%): 6.7
- Sample 2: n=24, Mean(ng/ml): 15.4, Standard deviation: 1.063, CV(%): 6.9
- Sample 3: n=24, Mean(ng/ml): 29.7, Standard deviation: 2.14, CV(%): 7.2
- 限制
- 仅限研究用
-
- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
-
: "Tissue and serum IGFBP7 protein as biomarker in high-grade soft tissue sarcoma." in: American journal of cancer research, Vol. 5, Issue 11, pp. 3446-54, (2016) (PubMed).
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: "Tissue and serum IGFBP7 protein as biomarker in high-grade soft tissue sarcoma." in: American journal of cancer research, Vol. 5, Issue 11, pp. 3446-54, (2016) (PubMed).
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- 抗原 See all IGFBP7 ELISA试剂盒
- IGFBP7 (Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))
- 别名
- IGFBP7 (IGFBP7 产品)
- 别名
- zgc:85888 ELISA Kit, AGM ELISA Kit, FSTL2 ELISA Kit, IBP-7 ELISA Kit, IGFBP-7 ELISA Kit, IGFBP-7v ELISA Kit, IGFBPRP1 ELISA Kit, MAC25 ELISA Kit, PSF ELISA Kit, RAMSVPS ELISA Kit, TAF ELISA Kit, Fstl2 ELISA Kit, Mac25 ELISA Kit, insulin like growth factor binding protein 7 ELISA Kit, insulin-like growth factor binding protein 7 ELISA Kit, IGFBP7 ELISA Kit, igfbp7 ELISA Kit, Igfbp7 ELISA Kit
- 背景
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Protein Function: Binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion. .
Background: Insulin-like growth factor-binding protein 7 is a protein that in humans is encoded by the IGFBP7 gene. The gene was mapped to chromosome 4q12. The major function of the protein is the regulation of availability of insulin-like growth factors(IGFs) in tissue as well as in modulating IGF binding to its receptors. IGFBP7 binds to IGF with high affinity. It also stimulates cell adhesion. The protein is implicated in some cancers.
Synonyms: Insulin-like growth factor-binding protein 7,IBP-7,IGF-binding protein 7,IGFBP-7,IGFBP-rP1,MAC25 protein,PGI2-stimulating factor,Prostacyclin-stimulating factor,Tumor-derived adhesion factor,TAF,IGFBP7,MAC25, PSF,
Full Gene Name: Insulin-like growth factor-binding protein 7
Cellular Localisation: Secreted. - 基因ID
- 3490
- UniProt
- Q16270
- 途径
- Growth Factor Binding
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