(6 references)Quick Overview for 人 IL-10 ELISpot Set (ABIN2690980)
抗原
See all IL-10 (IL10) 试剂盒适用
检测方法
应用范围
样品类型
-
-
实验类型
- Cell ELISA
-
品牌
- BD™ ELISPOT
-
产品特性
- The enzyme-linked immunospot (ELISPOT) assay is a powerful tool for detecting and enumerating individual cells that secrete a particular protein in vitro. Based on the sandwich ELISA, the ELISPOT assay derives its specificity and sensitivity by employing high affinity capture and detection antibodies and enzyme-amplification. Although originally developed for analyzing specific antibody-secreting cells, the assay has been adapted for measuring the frequencies of cells that produce and secrete other effector molecules, such as cytokines. The sensitivity of the assay lends itself to measurement of even very low frequencies of cytokine producing cells (e.g., 1/300,000). Unique strengths of the assay include high sensitivity, high throughput, high content analysis, minimal volume of biological material required, applicability to frozen/thawed biological samples, and compatibility with other assays. For example, cells analyzed by ELISPOT can be transferred for cloning, proliferation assays, flow cytometry, or other methods of analysis.
-
组件
-
10 ELISPOT plates
Unlabeled Capture Antibody
Biotinylated Detection Antibody
Enzyme Conjugate
Certificate of Analysis
-
-
Interleukin 10 (IL10) ELISA Kit
IL10 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 10 (IL10) ELISA KitIL10 适用: 人 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 10 (IL10) ELISA KitIL10 适用: 小鼠 AA 19-178 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Serum
Interleukin 10 (IL10) ELISA KitIL10 适用: 人 AA 19-178 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 10 (IL10) ELISA KitIL10 适用: 大鼠 AA 19-178 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum
Interleukin 10 (IL10) ELISA KitIL10 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 10 (IL10) ELISA KitIL10 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Ascitic Fluid, Cell Culture Supernatant, Cerebrospinal Fluid, Saliva, Serum, Urine
Interleukin 10 (IL10) ELISA KitIL10 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 10 (IL10) ELISA KitIL10 适用: 兔 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 10 (IL10) ELISA KitIL10 适用: 小鸡 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
-
-
应用备注
-
Unique strengths of the assay include the following:
High sensitivity
High throughput, high content analysis
Minimal volume of biological material required
Applicability to frozen/thawed biological samples
Compatibility with other assays. For example, cells analyzed by BD™ ELISPOT can be transferred for cloning, proliferation assays,flow cytometry, or other methods of analysis. -
说明
-
BD™ ELISPOT Human IL-10 ELISPOT Set - Reactivity Hu
-
板类型
- Uncoated
-
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C
-
储存方法
- Store unopened reagents at 2-8°C. Do not use reagents after expiration date, or if turbidity is evident.
-
-
-
: "A valid ELISPOT assay for enumeration of ex vivo, antigen-specific, IFNgamma-producing T cells." in: Journal of immunological methods, Vol. 227, Issue 1-2, pp. 99-107, (1999) (PubMed).
: "Enzyme-linked immunosorbent assay spot detection of interferon-gamma and interleukin 5-producing cells as a predictive marker for renal allograft failure." in: Transplantation, Vol. 66, Issue 2, pp. 219-24, (1998) (PubMed).
: "Regulation of mucosal and systemic antibody responses by T helper cell subsets, macrophages, and derived cytokines following oral immunization with live recombinant Salmonella." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 4, pp. 1504-14, (1996) (PubMed).
: "Cytokine-specific ELISPOT assay. Single cell analysis of IL-2, IL-4 and IL-6 producing cells." in: Journal of immunological methods, Vol. 160, Issue 2, pp. 181-9, (1993) (PubMed).
: "Reverse ELISPOT assay for clonal analysis of cytokine production. I. Enumeration of gamma-interferon-secreting cells." in: Journal of immunological methods, Vol. 110, Issue 1, pp. 29-36, (1988) (PubMed).
: "A solid-phase immunoenzymatic technique for the enumeration of specific antibody-secreting cells." in: Journal of immunological methods, Vol. 57, Issue 1-3, pp. 301-9, (1983) (PubMed).
-
: "A valid ELISPOT assay for enumeration of ex vivo, antigen-specific, IFNgamma-producing T cells." in: Journal of immunological methods, Vol. 227, Issue 1-2, pp. 99-107, (1999) (PubMed).
-
- IL-10 (IL10) (Interleukin 10 (IL10))
-
别名
- IL-10
抗原
-
