Serotonin ELISA 试剂盒
Quick Overview for Serotonin ELISA 试剂盒 (ABIN2648821)
抗原
See all Serotonin (5-HT) ELISA试剂盒适用
检测方法
应用范围
样品类型
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灵敏度
- 5 ng/mL
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产品特性
- Serotonin (5-Hydroxytryptamine), a biogenic amine, is a product of the tryptophan metabolism. It is a well evaluated neurotransmitter of the central nervous system and can be found in high concentrations in the chromaffine cells of the intestinal mucosa, in the platelets and the serotonergic neurones of the brain. Central-serotonergic neurons influence physiological functions such as sleep and the hormonal and cardio-vascular regulation. Increased serum levels can be found with malignant carcinoid, endogenous depression and schizophrenia.
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5-Hydroxytryptamine (5-HT) ELISA Kit
5-HT 适用: Various Species Colorimetric Competition ELISA 11.11 ng/mL - 900 ng/mL Plasma, Serum
5-Hydroxytryptamine (5-HT) ELISA Kit5-HT 适用: Various Species Colorimetric Competition ELISA 15.63 ng/mL - 1000 ng/mL Cell Culture Supernatant, Plasma, Serum
5-Hydroxytryptamine (5-HT) ELISA Kit5-HT 适用: Various Species Colorimetric Competition ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
5-Hydroxytryptamine (5-HT) ELISA Kit5-HT 适用: 小鼠 Colorimetric Competition ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
5-Hydroxytryptamine (5-HT) ELISA Kit5-HT 适用: 人 Colorimetric Competition ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
5-Hydroxytryptamine (5-HT) ELISA Kit5-HT 适用: 大鼠 Colorimetric Competition ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
5-Hydroxytryptamine (5-HT) ELISA Kit5-HT 适用: 绵羊 Colorimetric Competition ELISA 0.625 ng/mL - 10 ng/mL Plasma, Serum
5-Hydroxytryptamine (5-HT) ELISA Kit5-HT 适用: 大鼠 Colorimetric Competition ELISA 0.8-160 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 10 μL
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实验时间
- 2.5 h
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板类型
- Pre-coated
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实验流程
- The competitive Serotonin ELISA kit uses the microtiter plate format. Serotonin is bound to the solid phase of the microtiter plate. Acylated serotonin and solid phase bound serotonin compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase serotonin is detected by anti-rabbit/peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase serotonin is inversely proportional to the serotonin concentration of the sample.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- Serotonin (5-HT) (5-Hydroxytryptamine (5-HT))
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别名
- Serotonin
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物质类
- Chemical
抗原 See all Serotonin (5-HT) ELISA试剂盒
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