IL-3 ELISA 试剂盒
Quick Overview for IL-3 ELISA 试剂盒 (ABIN2648694)
抗原
See all IL-3 ELISA试剂盒适用
检测方法
应用范围
样品类型
-
-
原理
- Human Interleukin 3 (IL-3) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 3 (IL-3) concentrations in cell culture supernates, serum, and plasma.
-
Analytical Method
- Quantitative
-
灵敏度
- 15 pg/ml
-
产品特性
- Human Interleukin 3 (IL-3) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
-
-
Interleukin 3 (IL-3) ELISA Kit
IL-3 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 3 (IL-3) ELISA KitIL-3 适用: 小鸡 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 3 (IL-3) ELISA KitIL-3 适用: Cow Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 3 (IL-3) ELISA KitIL-3 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 3 (IL-3) ELISA KitIL-3 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 3 (IL-3) ELISA KitIL-3 适用: 犬 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Plasma, Serum
Interleukin 3 (IL-3) ELISA KitSmall Sample IL-3 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 3 (IL-3) ELISA KitIL-3 适用: 大鼠 AA 27-169 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Serum
Interleukin 3 (IL-3) ELISA KitIL-3 适用: 人 AA 20-152 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 3 (IL-3) ELISA KitIL-3 适用: 小鼠 AA 27-166 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Serum
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
样本量
- 100 μL
-
实验时间
- 3.5 h
-
板类型
- Pre-coated
-
实验流程
- Human Interleukin 3 (IL-3) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-3 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-3 is added to the wells and binds to the combination of capture antibody- IL-3 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-3 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-3 standard dilutions and IL-3 sample concentration determined.
-
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C
-
-
- IL-3 (Interleukin 3 (IL-3))
-
别名
- IL-3
-
途径
- JAK/STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy
抗原 See all IL-3 ELISA试剂盒
-
