IGFBP4 ELISA 试剂盒
Quick Overview for IGFBP4 ELISA 试剂盒 (ABIN2648687)
抗原
See all IGFBP4 ELISA试剂盒适用
检测方法
应用范围
-
-
原理
- IGFBP-4 ELISA Assay kit is an Enzyme-linked immunosorbent assay used for the quantitative and very sensitive determination of IGFBP-4 (Insulin-like Growth Factor Binding Protein 4) in biological fluids.
-
Analytical Method
- Quantitative
-
灵敏度
- 0.625 ng/ml
-
产品特性
- The IGFBP-4 ELISA Assay kit is for research use only and should not be used in diagnostic procedures.
-
-
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
样本量
- 50 μL
-
实验时间
- 2 h
-
板类型
- Pre-coated
-
实验流程
- The biotinylated antibody binds to the streptavidin coated microtiter plate. The IGFBP-4 of the standards/samples binds to this antibody. The biotinylated antibody and the standards/ samples are incubated on the microtiter plate at the same time. After a washing step the bound IGFBP-4, is then detected by a horseradish peroxidase-conjugated anti-IGFBP-4 antibody. After a second washing step the conjugate is added and incubated. The reaction with the substrate is stopped by addition of acid and the blue colour turns to yellow. The measured absorbance is directly proportional to the quantity of IGFBP-4.
-
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C
-
-
- IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))
-
别名
- IGFBP-4
-
途径
- WNT signaling, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process
抗原 See all IGFBP4 ELISA试剂盒
-