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IGFBP4 ELISA 试剂盒

This 人 IGFBP4 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 IGFBP4.
产品编号 ABIN2648687
发货至: 中国

Quick Overview for IGFBP4 ELISA 试剂盒 (ABIN2648687)

抗原

See all IGFBP4 ELISA试剂盒
IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))

适用

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检测方法

Colorimetric

应用范围

ELISA
  • 原理

    IGFBP-4 ELISA Assay kit is an Enzyme-linked immunosorbent assay used for the quantitative and very sensitive determination of IGFBP-4 (Insulin-like Growth Factor Binding Protein 4) in biological fluids.

    Analytical Method

    Quantitative

    灵敏度

    0.625 ng/ml

    产品特性

    The IGFBP-4 ELISA Assay kit is for research use only and should not be used in diagnostic procedures.
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    样本量

    50 μL

    实验时间

    2 h

    板类型

    Pre-coated

    实验流程

    The biotinylated antibody binds to the streptavidin coated microtiter plate. The IGFBP-4 of the standards/samples binds to this antibody. The biotinylated antibody and the standards/ samples are incubated on the microtiter plate at the same time. After a washing step the bound IGFBP-4, is then detected by a horseradish peroxidase-conjugated anti-IGFBP-4 antibody. After a second washing step the conjugate is added and incubated. The reaction with the substrate is stopped by addition of acid and the blue colour turns to yellow. The measured absorbance is directly proportional to the quantity of IGFBP-4.

    限制

    仅限研究用
  • 储存条件

    4 °C
  • 抗原 See all IGFBP4 ELISA试剂盒

    IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))

    别名

    IGFBP-4

    途径

    WNT signaling, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process
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