Interferon gamma ELISA 试剂盒
Quick Overview for Interferon gamma ELISA 试剂盒 (ABIN2648682)
抗原
See all Interferon gamma (IFNG) ELISA试剂盒适用
检测方法
应用范围
样品类型
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原理
- Mouse Interferon Gamma (IFN-γ) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of mouse Interferon Gamma (IFN-γ) concentrations in cell culture supernates, serum, and plasma.
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Analytical Method
- Quantitative
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灵敏度
- 7 pg/ml
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产品特性
- Mouse Interferon Gamma (IFN-γ) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
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Interferon gamma (IFNG) ELISA Kit
IFNG 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: 小鸡 Colorimetric Competition ELISA 3.125 pg/mL - 800 pg/mL Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: 小鼠 AA 23-155 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Serum
Interferon gamma (IFNG) IQ-ELISA KitIFNG 适用: 人 qPCR Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
Interferon gamma (IFNG) ELISA KitIFNG 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: Pig Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: 人 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Cell Culture Supernatant, Cerebrospinal Fluid, Serum, Tissue Homogenate, Urine
Interferon gamma (IFNG) ELISA KitIFNG 适用: 兔 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 100 μL
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实验时间
- 3 h
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板类型
- Pre-coated
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实验流程
- Mouse Interferon Gamma (IFN-γ) ELISA Assay Kit the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IFN-γ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-γ present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IFN-γ is added to the wells and binds to the combination of capture antibody-IFN-γ in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IFN-γ present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IFN-γ standard dilutions and IFN-γ sample concentration determined.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- Interferon gamma (IFNG)
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别名
- IFN-gamma
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途径
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
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