Histamine ELISA 试剂盒
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Quick Overview for Histamine ELISA 试剂盒 (ABIN2648672)
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See all Histamine (HIS) ELISA试剂盒适用
检测方法
应用范围
样品类型
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灵敏度
- 0.02 ng/mL
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产品特性
- Histamine plays a major role in the initial phase of an anaphylactic reaction. The quantification of histamine in plasma after allergen administration is of clinical interest. Histamine is part of the immune response to foreign pathogens and it increases the permeability of the capillaries to white blood cells and other proteins in order to allow them to engage foreign invaders in the affected tissues. Responsible for the biological effects of histamine in tissue are the activation of different surface receptors, for instance H1, H2 and H3. Histamine is involved in the regulating physiological function in the gut and acting as a neurotransmitter.
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Histamine (HIS) ELISA Kit
HIS 适用: Various Species Colorimetric Competition ELISA 1.23 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Histamine (HIS) ELISA KitHIS 适用: 小鼠 Colorimetric Competition ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Histamine (HIS) ELISA KitHIS 适用: 人 Colorimetric Competition ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Histamine (HIS) ELISA KitHIS 适用: 兔 Colorimetric Competition ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Histamine (HIS) ELISA KitHIS 适用: 化学剂 Colorimetric Competition ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Histamine (HIS) ELISA KitHIS 适用: Various Species Colorimetric Sandwich ELISA 7.8-500 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Histamine (HIS) ELISA KitHIS 适用: 化学剂 Colorimetric Competition ELISA 5.0-100 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Histamine (HIS) ELISA KitHIS 适用: 小鼠 Colorimetric Competition ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 50 μL
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实验时间
- 2.5 h
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板类型
- Pre-coated
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实验流程
- The competitive Histamine ELISA kit uses the microtiter plate format. Histamine is bound to the solid phase of the microtiter plate. Acylated histamine and solid phase bound histamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase histamine is detected by anti-rabbit/peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase histamine is inversely proportional to the histamine concentration of the sample.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- Histamine (HIS)
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别名
- Histamine
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物质类
- Chemical
抗原 See all Histamine (HIS) ELISA试剂盒
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