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Histamine ELISA 试剂盒

This 人 Histamine ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 Histamine.

产品编号 ABIN2648672

发货至: 中国

Quick Overview for Histamine ELISA 试剂盒 (ABIN2648672)

抗原

See all Histamine (HIS) ELISA试剂盒

Histamine (HIS)

适用

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人

检测方法

Colorimetric

应用范围

ELISA

样品类型

Plasma, Urine, Cell Culture Cells

Discover our top product HIS ELISA Kit

灵敏度

0.02 ng/mL

产品特性

Histamine plays a major role in the initial phase of an anaphylactic reaction. The quantification of histamine in plasma after allergen administration is of clinical interest. Histamine is part of the immune response to foreign pathogens and it increases the permeability of the capillaries to white blood cells and other proteins in order to allow them to engage foreign invaders in the affected tissues. Responsible for the biological effects of histamine in tissue are the activation of different surface receptors, for instance H1, H2 and H3. Histamine is involved in the regulating physiological function in the gut and acting as a neurotransmitter.
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Histamine (HIS) ELISA Kit
HIS 适用: 兔 Colorimetric Competition ELISA 5.0-100 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

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HIS 适用: 豚鼠 Colorimetric Competition ELISA 5.0-100 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

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HIS 适用: 大鼠 Colorimetric Competition ELISA 5.0-100 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

应用备注

Optimal working dilution should be determined by the investigator.

样本量

50 μL

实验时间

2.5 h

板类型

Pre-coated

实验流程

The competitive Histamine ELISA kit uses the microtiter plate format. Histamine is bound to the solid phase of the microtiter plate. Acylated histamine and solid phase bound histamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase histamine is detected by anti-rabbit/peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase histamine is inversely proportional to the histamine concentration of the sample.

限制

仅限研究用
储存条件

4 °C
抗原 See all Histamine (HIS) ELISA试剂盒

Histamine (HIS)

别名

Histamine

物质类

Chemical

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