CD44 ELISA 试剂盒
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Quick Overview for CD44 ELISA 试剂盒 (ABIN2648547)
抗原
See all CD44 ELISA试剂盒适用
检测方法
应用范围
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原理
- CD44 ELISA is to be used for the in-vitro quantitative determination of soluble CD44 (CD44/phagocytic glycoprotein-1/Pgp-1) in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human CD44.
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Analytical Method
- Quantitative
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产品特性
- Human CD44 ELISA Assay kit
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CD44 (CD44) ELISA Kit
CD44 适用: 人 Colorimetric Sandwich ELISA 78-5000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
CD44 (CD44) ELISA KitCD44 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD44 (CD44) ELISA KitCD44 适用: 大鼠 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
CD44 (CD44) ELISA KitCD44 适用: 大鼠 AA 22-271 Colorimetric Sandwich ELISA 312-20.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
CD44 (CD44) ELISA KitCD44 适用: 小鼠 AA 25-224 Colorimetric Sandwich ELISA 312-20.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
- The CD44 Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for CD44 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD44 concentrations and unknowns are pipetted into these wells. During the first incubation, the CD44 antigen, standards and zeros are bound this is followed by a separate incubation where the biotinylated monoclonal antibody specific for CD44 is added. After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD44 present in the samples.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- CD44
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别名
- CD44 / Pgp-1
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途径
- Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling
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