IL4 Receptor ELISA 试剂盒
Quick Overview for IL4 Receptor ELISA 试剂盒 (ABIN2648537)
抗原
See all IL4 Receptor (IL4R) ELISA试剂盒适用
检测方法
应用范围
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原理
- Human IL-4R ELISA is to be used for the in-vitro quantitative determination of soluble IL-4 receptor molecule (CD124) in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human IL-4R.
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Analytical Method
- Quantitative
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产品特性
- Human IL-4R ELISA Assay kit is for Research Use Only.
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
- The IL-4R Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for IL-4R has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-4R concentrations and unknowns are pipetted into these wells. During the first incubation, the IL-4R antigen and a biotinylated monoclonal antibody specific for IL-4R are simultaneously incubated. After washing, the enzyme ( streptavidin-peroxydase ) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-4R present in the samples.
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限制
- 仅限研究用
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储存条件
- 4 °C
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- IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))
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别名
- CD124 / IL-4R
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途径
- JAK/STAT Signaling, Positive Regulation of Immune Effector Process
抗原 See all IL4 Receptor (IL4R) ELISA试剂盒
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