IL4 Receptor ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for IL4 Receptor ELISA 试剂盒 (ABIN6730183)
抗原
See all IL4 Receptor (IL4R) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 1.33 ng/mL
-
原理
- Mouse IL-4 R alpha ELISA Kit for Plasma, Serum, and Cell Culture Supernatants samples.
-
Analytical Method
- Quantitative
-
特异性
- This ELISA antibody pair detects mouse IL-4 R alpha. Other species not determined.
-
产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
-
组件
-
- Pre-Coated 96-well Strip Microplate
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- Wash Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
- Assay Diluent(s)
-
试剂未包括
-
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for ELISA data analysis
- Tubes to prepare standard and sample dilutions
-
-
Interleukin 4 Receptor (IL4R) ELISA Kit
IL4R 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 4 Receptor (IL4R) ELISA KitIL4R 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 4 Receptor (IL4R) ELISA KitIL4R 适用: 小鼠 AA 26-233 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 4 Receptor (IL4R) ELISA KitIL4R 适用: 人 AA 26-232 Colorimetric Sandwich ELISA 46.9 pg/mL - 3000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 4 Receptor (IL4R) ELISA KitIL4R 适用: 马 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 4 Receptor (IL4R) ELISA KitIL4R 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 4 Receptor (IL4R) ELISA KitIL4R 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
-
应用备注
- Suggested Plasma Dilution: 2 fold
-
样本量
- 100 μL
-
板类型
- Pre-coated
-
实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature.
- Add 100 μL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
- Add 100 μL prepared Streptavidin solution to each well. Incubate 45 minutes at room temperature.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately
-
限制
- 仅限研究用
-
-
-
储存条件
- -20 °C
-
储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
-
有效期
- 6 months
-
-
- IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))
-
别名
- IL-4 R alpha
-
背景
- Gene Symbols: Interleukin-4 receptor subunit alpha (IL-4 receptor subunit alpha) (IL-4R subunit alpha) (IL-4R-alpha) (IL-4RA) (CD antigen CD124) [Cleaved into: Soluble interleukin-4 receptor subunit alpha (Soluble IL-4 receptor subunit alpha) (Soluble IL-4R-alpha) (sIL4Ralpha/prot) (IL-4-binding protein) (IL4-BP)
-
UniProt
- P16382
-
途径
- JAK/STAT Signaling, Positive Regulation of Immune Effector Process
抗原 See all IL4 Receptor (IL4R) ELISA试剂盒
-
