KIT ELISA 试剂盒
Quick Overview for KIT ELISA 试剂盒 (ABIN2539205)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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原理
- Rat Mast/Stem Cell Growth Factor Receptor Kit (KIT) ELISA Kit
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Analytical Method
- Quantitative
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灵敏度
- 18.8 pg/mL
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组件
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The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
- Pre-coated 96-Well Microplate
- Standard
- Standard Diluent Buffer
- Wash Buffer
- Detection Reagent A
- Detection Reagent B
- Diluent A
- Diluent B
- TMB Substrate
- Stop Solution
- Plate Sealer
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试剂未包括
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- 37 °C incubator
- Multi and single channel pipettes and sterile pipette tips
- Squirt bottle or automated microplate washer
- 1.5 mL tubes
- Distilled water
- Absorbent filter papers
- 100 mL and 1 liter graduated cylinders
- Microplate reader (wavelength: 450 nm)
- ELISA Shaker
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Mast/stem Cell Growth Factor Receptor (KIT) ELISA Kit
KIT 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Mast/stem Cell Growth Factor Receptor (KIT) ELISA KitKIT 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Mast/stem Cell Growth Factor Receptor (KIT) ELISA KitKIT 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Mast/stem Cell Growth Factor Receptor (KIT) ELISA KitKIT 适用: 人 AA 26-520 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Mast/stem Cell Growth Factor Receptor (KIT) ELISA KitKIT 适用: 小鼠 AA 25-527 Colorimetric Sandwich ELISA
Mast/stem Cell Growth Factor Receptor (KIT) ELISA KitKIT 适用: 小鼠 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal dilutions/concentrations should be determined by the end user.
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说明
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The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
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板类型
- Pre-coated
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实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Mast/Stem Cell Growth Factor Receptor Kit (KIT) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Mast/Stem Cell Growth Factor Receptor Kit (KIT) were tested on 3 different plates, 8 replicates in each plate.
CV (%) = (Standard Deviation / mean) x 100
Intra-Assay: CV<10%
Inter-Assay: CV<10%
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
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有效期
- 6 months
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- KIT (Mast/stem Cell Growth Factor Receptor (KIT))
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别名
- Stem Cell Factor Receptor
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity
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