Interferon gamma ELISA 试剂盒
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Quick Overview for Interferon gamma ELISA 试剂盒 (ABIN2111992)
抗原
See all Interferon gamma (IFNG) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 50 pg/mL
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灵敏度
- < 7.5 pg/mL
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组件
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MOUSE IFn-y MICROTITER PLATE
MOUSE IFn-y BIOTIN CONJUGATE
AVIDIN HRP CONJUGATE
MOUSE IFn-y STANDARD
CALIBRATOR DILUENT I
CALIBRATOR DILUENT II
WASH BUFFER (20X/96 wells, 30X/192 wells)
SUBSTRATE A
SUBSTRATE B
STOP SOLUTION
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Interferon gamma (IFNG) ELISA Kit
IFNG 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: 小鸡 Colorimetric Competition ELISA 3.125 pg/mL - 800 pg/mL Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: 小鼠 AA 23-155 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Serum
Interferon gamma (IFNG) IQ-ELISA KitIFNG 适用: 人 qPCR Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
Interferon gamma (IFNG) ELISA KitIFNG 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: Pig Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interferon gamma (IFNG) ELISA KitIFNG 适用: 人 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Cell Culture Supernatant, Cerebrospinal Fluid, Serum, Tissue Homogenate, Urine
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板类型
- Pre-coated
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实验流程
- This mouse IFN-y ELISA is a 4.5 hour solid phase immunoassay readily applicable to measure IFN-y levels in mouse serum, cell culture supernatant, and other biological fluids in the range of 0 to 3200pg/ml. It is expected to be useful for investigations into the relationship between IFN-y and various diseases.This mouse IFN-y enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre- coated with a monoclonal specific for mouse IFN-u. Standards or samples are then added to the appropriate microtiter plate wells and incubated. In order to quantitatively determine the amount of mouse IFN-u present in the sample, a biotin-conjugated antibody specific for mouse IFN-y is added to each well to "sandwich" the IFN-u immobilized during the first incubation. The microtiter plate then undergoes a second incubation and the wells are thoroughly washed to remove all unbound Biotin-conjugated antibodies. Avidin can bind to biotin with high affinity and amplify the biotin signal. A preparation of avidin conjugated HRP is added to each well and incubated. The avidin HRP conjugate will bind to wells containing biotin. After washing the wells, the unbounded avidin-HRP conjugate is removed. A TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IFN-y and biotin-conjugated antibody, avidin conjugated HRP will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.
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限制
- 仅限研究用
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- Interferon gamma (IFNG)
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途径
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
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