TICAM1 ELISA 试剂盒
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Quick Overview for TICAM1 ELISA 试剂盒 (ABIN2044679)
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检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 62.5 pg/mL
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灵敏度
- < 22.9 pg/mL
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板类型
- Pre-coated
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实验流程
- Toll Like Receptor Adaptor Molecule 1 (TICAM1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Toll Like Receptor Adaptor Molecule 1 (TICAM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Toll Like Receptor Adaptor Molecule 1 (TICAM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Toll Like Receptor Adaptor Molecule 1 (TICAM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Toll Like Receptor Adaptor Molecule 1 (TICAM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- TICAM1 (Toll-Like Receptor Adaptor Molecule 1 (TICAM1))
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别名
- Toll Like Receptor Adaptor Molecule 1 (TICAM1)
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途径
- TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades
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