RAC1 ELISA 试剂盒
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Quick Overview for RAC1 ELISA 试剂盒 (ABIN2044385)
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检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.15 ng/mL
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灵敏度
- < 0.060 ng/mL
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RAC1 ELISA Kit
适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
RAC1 ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
RAC1 ELISA Kit适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
RAC1 ELISA Kit适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
RAC1 ELISA Kit适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
RAC1 ELISA Kit适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
RAC1 ELISA Kit适用: 小鼠 Colorimetric Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ras Related C3 Botulinum Toxin Substrate 1 (Rac1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ras Related C3 Botulinum Toxin Substrate 1 (Rac1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ras Related C3 Botulinum Toxin Substrate 1 (Rac1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- RAC1
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别名
- Ras Related C3 Botulinum Toxin Substrate 1 (Rac1)
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途径
- WNT signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Thromboxane A2 Receptor Signaling, VEGF Signaling
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