PDK1 ELISA 试剂盒
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Quick Overview for PDK1 ELISA 试剂盒 (ABIN2044369)
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检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 1.56 ng/mL
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灵敏度
- < 0.58 ng/mL
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Pyruvate Dehydrogenase Kinase 1 (PDK1) ELISA Kit
PDK1 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Pyruvate Dehydrogenase Kinase 1 (PDK1) ELISA KitPDK1 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))
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别名
- Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1)
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途径
- PI3K-Akt Signaling, RTK signaling, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Warburg Effect
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