NOTCH2 ELISA 试剂盒
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Quick Overview for NOTCH2 ELISA 试剂盒 (ABIN2044111)
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检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 0.78 ng/mL
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灵敏度
- < 0.33 ng/mL
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Notch 2 (NOTCH2) ELISA Kit
NOTCH2 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Lysate, Tissue Homogenate
Notch 2 (NOTCH2) ELISA KitNOTCH2 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Notch 2 (NOTCH2) ELISA KitNOTCH2 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Notch Homolog 2 (NOTCH2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Notch Homolog 2 (NOTCH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Notch Homolog 2 (NOTCH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Notch Homolog 2 (NOTCH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Notch Homolog 2 (NOTCH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- NOTCH2 (Notch 2 (NOTCH2))
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别名
- Notch Homolog 2 (NOTCH2)
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途径
- Notch Signaling, Stem Cell Maintenance
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