MAP1LC3A ELISA 试剂盒
Quick Overview for MAP1LC3A ELISA 试剂盒 (ABIN2043973)
抗原
See all MAP1LC3A ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 1.56 ng/mL
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灵敏度
- < 0.59 ng/mL
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Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) ELISA Kit
MAP1LC3A 适用: 人 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) ELISA KitMAP1LC3A 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) ELISA KitMAP1LC3A 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) ELISA KitMAP1LC3A 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
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板类型
- Pre-coated
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实验流程
- Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))
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别名
- Microtubule Associated Protein 1 Light Chain 3 alpha (MAP1LC3a)
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途径
- Autophagy
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