JAK3 ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for JAK3 ELISA 试剂盒 (ABIN2043827)
抗原
See all JAK3 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
-
-
最低检测浓度
- 0.15 ng/mL
-
灵敏度
- < 0.066 ng/mL
-
-
Janus Kinase 3 (JAK3) ELISA Kit
JAK3 适用: 大鼠 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Janus Kinase 3 (JAK3) ELISA KitJAK3 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Janus Kinase 3 (JAK3) ELISA KitJAK3 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Janus Kinase 3 (JAK3) ELISA KitJAK3 适用: 大鼠 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cell Lysate, Tissue Homogenate
-
-
板类型
- Pre-coated
-
实验流程
- Janus Kinase 3 (JAK3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Janus Kinase 3 (JAK3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Janus Kinase 3 (JAK3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Janus Kinase 3 (JAK3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Janus Kinase 3 (JAK3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
-
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C/-20 °C
-
-
- JAK3 (Janus Kinase 3 (JAK3))
-
途径
- JAK/STAT Signaling, RTK signaling, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus, Activated T Cell Proliferation, Unfolded Protein Response
抗原 See all JAK3 ELISA试剂盒
-
